Long-read nanopore sequencing validated for HLA-class I typing in routine diagnostics

Matching of HLA gene polymorphisms by high-resolution DNA sequence analysis is gold standard for determining compatibility between patient and donor for hematopoietic stem cell transplantation. Single molecule sequencing (PacBio or MinION) is a newest (third) generation sequencing approach. MinION is a nanopore sequencing platform, which provides long targeted DNA sequences. The long reads provide unambiguous phasing, but the initial high error profile prevented its use in high-impact applications such as Human Leukocyte Antigen (HLA) typing for HLA matching of donor and recipient in the transplantation setting.

Source: Journal of Molecular Diagnostics - April 13, 2020 Category: Pathology Authors: Benedict M. Matern, Timo I. Olieslagers, Mathijs Groeneweg, Burcu Duygu, Lotte Wieten, Marcel G.J. Tilanus, Christina E.M. Voorter Source Type: research