New PA/1220/98-like variant of infectious bronchitis virus in Poland.

New PA/1220/98-like variant of infectious bronchitis virus in Poland. Avian Pathol. 2020 Apr 09;:1-25 Authors: Domanska-Blicharz K, Sajewicz-Krukowska J, Lisowska A Abstract The aim of the present study was to report the first detection of a new IBV variant in Polish commercial flocks which is completely different to any previously known in this region. In 2018, samples from Ross 308 breeding hens aged 35 weeks were delivered for IBV diagnosis. IBV presence was detected, but all attempts to amplify the S gene fragment were negative. The field material was analysed using the Illumina MiSeq platform and a 1073-nt fragment of the S1 coding region was obtained. The gCoV/ck/Poland/516/2018 strain shared only 52.7-58.1% nucleotide identity to any known genotypes of IBV and shared the highest identity of 81.4% to the unique North American PA/1220/98 variant. Based on the obtained sequence, a specific molecular test was constructed and used for screening of chicken samples from 35 field cases delivered to our laboratory between 2018 and 2019 for IBV diagnosis. Application of this test enabled detection of another three chicken flocks as positive for this new strain. All positives were identified in commercial layers with egg production problems. To date, the virus has not been detected in broiler chickens.Taking into account the proposed criteria for the definition of a new IBV genotype or lineage, it seems that the detected viruses in Polan...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research