Antibacterial activity of SPIONs versus ferrous and ferric ions under aerobic and anaerobic conditions: a preliminary mechanism study

In this study, the authors evaluated the antibacterial activity of magnetic NPs in comparison with ferrous and ferric ions. The level of reactive oxygen species (ROS) in the treated Staphylococcus aureus and Escherichia coli bacteria were directly measured by fluorometric detection. The results showed that iron ions and SPIONs had significant dependent antimicrobial activities. SPIONs showed greater inhibitory effects than ferrous and ferric ions against the growth of treated bacterial strains under anaerobic conditions, while in aerobic conditions, ferrous showed the strongest antibacterial activity. In anaerobic conditions, they observed the greatest ROS formation and lowest minimum inhibitory concentration in the SPION-treated group in comparison with the other groups. It seems that the release of iron ions from SPIONs and subsequent activation of ROS pathway are the main antibacterial mechanisms of action. Nevertheless, the greater antibacterial effect of SPIONs in anaerobic conditions represents other mechanisms involved in the antibacterial activity of these NPs
Source: IET Nanobiotechnology - Category: Nanotechnology Source Type: research

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Subgaleal hematoma is an uncommon, but potential sequela of birth trauma and instrument-assisted delivery of neonates, as well as head trauma in young children. A rare complication is an infection of the subgaleal hematoma, which typically happens due to concomitant scalp lacerations. Escherichia coli is the most common causative pathogen in peripartum cases, and Staphylococcus aureus predominates in trauma cases. An even more rare complication is infection of the hematoma with intact overlying skin, the proposed mechanism of action of which is a hematogenous spread of the bacteria.
Source: The American Journal of Emergency Medicine - Category: Emergency Medicine Authors: Source Type: research
The objective of this study was to characterize the response to an aerosolized lysate of killed bacteria, as a basis ...
Source: BMC Veterinary Research - Category: Veterinary Research Authors: Tags: Research article Source Type: research
Sphingosine is a long-chain sphingoid base that has been shown to have bactericidal activity against many pathogens, including Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. We have previously demonstrated that sphingosine is present in nasal, tracheal, and bronchial epithelial cells and constitutes a central element of the defense of the airways against bacterial pathogens. Here, using assorted lipid-binding and cell biology assays, we demonstrate that exposing P. aeruginosa and S. aureus cells to sphingosine results in a very rapid, i.e. within minutes, permeabilization of the bacterial plasma membr...
Source: Journal of Biological Chemistry - Category: Chemistry Authors: Tags: Lipids Source Type: research
This study provided evidence that the plant has antibacterial, antioxidant, and antidiabetic properties. PMID: 32454801 [PubMed - in process]
Source: The Scientific World Journal - Category: Science Tags: ScientificWorldJournal Source Type: research
Abstract Traditional solutions of food packing are limited for antimicrobial packaging materials that interact with the product to inhibit the growth of microorganisms. In this work, adhesive composite was developed as an active packaging material using allyl isothiocyanate (AIC) in vapor phase. Such adhesive composite was made of gelatin electrospun fibers loaded by AIC. Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced at 15 kV, 15 cm air gap and 0.2 mL/h flow rate. This mat was supported by pressure-sensitive adhesive (PSA) made of hydroxyethyl cellulose bac...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
Abstract In this work, we have prepared cerium oxide (CeO2) nanoparticles (NPs) by laser ablation in water at different laser energies. The structural and optical properties of synthesized nanoparticles were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), Raman spectroscopy, energy dispersive X-ray (EDX), and UV-Vis absorption. XRD results confirmed that the synthesized cerium oxide NPs were crystalline in nature with cubic structure. SEM investigations show that the nanoparticles having a spherical shape with diameter ranged from 26 to 37 nm depending on the laser energy. The an...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research
This study aimed to determine the centesimal composition and physicochemical characteristics of the pulp and seed of S. cumini (L.) Skeels, as well as the content of total phenolic compounds and the antioxidant, antibacterial, antibiofilm and anti-quorum sensing (QS) activities of the phenolic extracts obtained from the pulp and the seeds of this fruit. The in vitro antibacterial and anti-QS activities of active films incorporating phenolic extracts were also evaluated. Additionally, we performed molecular docking of phenolic compounds present in jambolan with the CviR QS regulator of Chromobacterium violaceum. The composi...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusions. Together with its previously shown anti-inflammatory properties, the bactericidal activity of CPZO gives it the potential to be a first-line therapeutic option for chronic various ulcers and, possibly, other chronic ulcers, preventing or controlling microbial infections, and leading to the healing of such complicated chronic ulcers. PMID: 32459619 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
Abstract Toxin-antitoxin (TA) systems are ubiquitously found in bacteria and are related to cell maintenance and survival under environmental stresses such as heat shock, nutrient starvation, and antibiotic treatment. Here, we report for the first time the crystal structure of the Staphylococcus aureus TA complex YoeBSa1-YefMSa1 at a resolution of 1.7 Å. This structure reveals a heterotetramer with a 2:2 stoichiometry between YoeBSa1 and YefMSa1. The N-terminal regions of the YefMSa1 antitoxin form a homodimer characteristic of a hydrophobic core, and the C-terminal extended region of each YefMSa1 proto...
Source: Biochemical and Biophysical Research communications - Category: Biochemistry Authors: Tags: Biochem Biophys Res Commun Source Type: research
We present Prokaryotic Expression-profiling by Tagging RNA In Situ and sequencing (PETRI-seq), a high-throughput prokaryotic scRNA-seq pipeline. We demonstrated that PETRI-seq effectively barcoded single bacterial cells in a species-mixing experiment with E. coli (MG1655) and S. aureus (USA300). Within the S. aureus population, we found rare prophage induction in 0.04% of cells. We further demonstrated that PETRI-seq was able to distinguish between E. coli growth phases based on mRNA expression patterns by combining stationary E. coli with exponential E. coli in multiple experiments.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Escherichia coli Staphylococcus aureus Source Type: research
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