In-vitro and in-vivo evaluation of chitosan-based thermosensitive gel containing lorazepam NLCs for the treatment of status epilepticus

The objective of this study was to develop an in-situ gel containing lorazepam (LZM) loaded nanostructured lipid carriers (NLCs) for direct nose-to-brain delivery in order to increase drug therapeutic efficacy in the treatment of epilepsy. Accordingly, LZM loaded NLCs were formulated using emulsification solvent diffusion and evaporation method; then the effects of the formulation variables on different physicochemical characteristics of NLCs were investigated. Thermosensitive in-situ gels containing LZM-NLCs were prepared using a combination of chitosan and β-glycerol phosphate (β-GP). The anticonvulsant efficacy of LZM-NLCs-Gel was then examined using the pentylenetetrazole (PTZ) model. The optimised NLCs were spherical, showing the particle size of 71.70 ± 5.16 nm and the zeta potential of −20.06 ± 2.70 mV. The pH and gelation time for the chitosan solution with 15% (w/v) β-GP were determined to be 7.12 ± 0.03 and 5.33 ± 0.58 min, respectively. The in-vivo findings showed that compared with the control group and the group that received LZM-Gel, the occurrence of PTZ-induced seizures in the rats was significantly reduced by LZM-NLCs-Gel after intranasal administration. These results, therefore, suggested that the LZM-NLCs-Gel system could have potential applications for brain targeting through nasal route and might increase LZM therapeutic efficacy in the treatment...
Source: IET Nanobiotechnology - Category: Nanotechnology Source Type: research

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Publication date: September 2020Source: Epilepsy &Behavior, Volume 110Author(s): Christopher Ll Morgan, Stefan Varga, Wan Tsong, Sara Jenkins-Jones, Sarah Holden
Source: Epilepsy and Behavior - Category: Neurology Source Type: research
The recent study by Vollono and colleagues [1] provides a concise report on a SARS-CoV-2 associated focal status epilepticus case. The authors hypothesize that a neurotropic mechanism may be involved in the development of the clinical presentation, albeit without PCR -proven neuroinvasion.
Source: Seizure: European Journal of Epilepsy - Category: Neurology Authors: Tags: Letter to the Editor Source Type: research
Conditions:   Resistant Epilepsy, Drug;   Adolescent Epilepsy;   Children Epilepsy;   Children and Adolescents With Resistant Epilepsies Interventions:   Drug: MGCND00EP1;   Drug: Placebo;   Diagnostic Test: ECG;   Diagnostic Test: EEG;   Diagnostic Test: Blood and urine collection Sponsor:   MGC Pharmaceuticals d.o.o Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
To report progress, to identify gaps, and to plan epilepsy surveillance and research activities more effectively, the Centers for Disease Control and Prevention (CDC) Epilepsy Program has summarized findings from selected CDC-supported surveillance and epi...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Ergonomics, Human Factors, Anthropometrics, Physiology Source Type: news
Abstract SUMMARYTaenia solium neurocysticercosis (NCC) is endemic in most of the world and contributes significantly to the burden of epilepsy and other neurological morbidity. Also present in developed countries because of immigration and travel, NCC is one of few diseases targeted for eradication. This paper reviews all aspects of its life cycle (taeniasis, porcine cysticercosis, human cysticercosis), with a focus on recent advances in its diagnosis, management, and control. Diagnosis of taeniasis is limited by poor availability of immunological or molecular assays. Diagnosis of NCC rests on neuroimaging finding...
Source: Clinical Microbiology Reviews - Category: Microbiology Authors: Tags: Clin Microbiol Rev Source Type: research
Conditions:   Resistant Epilepsy, Drug;   Adolescent Epilepsy;   Children Epilepsy;   Children and Adolescents With Resistant Epilepsies Interventions:   Drug: MGCND00EP1;   Drug: Placebo;   Diagnostic Test: ECG;   Diagnostic Test: EEG;   Diagnostic Test: Blood and urine collection Sponsor:   MGC Pharmaceuticals d.o.o Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Epilepsy death rates are still high among young adults and have not declined over time despite treatment advances, researchers say.Medscape Medical News
Source: Medscape Neurology and Neurosurgery Headlines - Category: Neurology Tags: Neurology & Neurosurgery News Source Type: news
NEUROSURGERY CLINICS OF NORTH AMERICA
Source: Neurosurgery Clinics of North America - Category: Neurosurgery Authors: Source Type: research
The notion of paradigm shifts in science was introduced in 1962 by the American physicist and philosopher Thomas Kuhn, describing a set of fundamental changes in the basic concepts, and subsequently, practices, of a scientific discipline. During the last decades, the field of epilepsy surgery in the United States has been through what Kuhn termed a revolutionary period; a period during which a paradigm shift in the way we comprehend and address surgically treatable epilepsies is taking place within our scientific and clinical community.
Source: Neurosurgery Clinics of North America - Category: Neurosurgery Authors: Source Type: research
The need to emphasize the network concept arises from the fact that the traditional surgical philosophy in American epilepsy centers has prioritized an electrical-anatomic, focus-oriented approach. In contrast, the stereoelectroencephalography philosophy focuses on using electrophysiology to determine the regions of cortex generating the clinical manifestation of the seizure. Viewing epilepsy surgery as network surgery enables optimal consideration of decisions related to the need for and method of intracranial monitoring, potential role of subcortical structures in seizure organization and propagation, upfront use of comb...
Source: Neurosurgery Clinics of North America - Category: Neurosurgery Authors: Source Type: research
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