Development of a Rapid and Sensitive IDH1/2 Mutation Detection Method for Glial Tumors and a Comparative Mutation Analysis of 236 Glial Tumor Samples

ConclusionSanger sequencing and IHC-based methods have shortcomings when detecting mutations in glial tumors so can missIDH1/2 mutations in glial tumors when used alone without proper modifications. 3m-ARMS-based mutation detection is fast and simple with potential for use as a diagnostic test for the majority of hot spot mutations inIDH1/2 genes. It can detectIDH1/2 mutations within an hour so can be adapted for intraoperative diagnosis.
Source: Molecular Diagnosis and Therapy - Category: Molecular Biology Source Type: research