GSE148288 A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: the transcription factor CgTog1

Contributors : Pedro Pais ; Susana Vagueiro ; Dalila Mil-Homens ; Andreia I Pimenta ; Romeu Viana ; Michiyo Okamoto ; Hiroji Chibana ; Ars énio M Fialho ; Miguel C TeixeiraSeries Type : Expression profiling by high throughput sequencingOrganism : [Candida] glabrataThe goals of this study are to compare C. glabrata transcriptome profiling (RNA-seq) upon exposure to hydrogen peroxide in order to assess the correspondent response. The role of the transcription factor Tog1 is clarified through comparison of the transcrptome profiles of WT and Tog1 mutant cells. mRNA profiles of WT and ∆tog1 were generated by deep sequencing, in duplicate, using Illumina HiSeq. The sequence reads that passed quality filters were analyzed with TopHat followed by HTSeq. RNA-seq data allowed to identify genes whose expression is regulated by the Tog1 transcription factor (147 positively regulated a nd 112 negatively regulated) with a log2fold change ≥0.5 and p value
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing [Candida] glabrata Source Type: research

Related Links:

Publication date: Available online 4 June 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Thomas J. Dauben, Carolin Dewald, Izabela Firkowska-Boden, Christian Helbing, Henrik Peisker, Martin Roth, Jörg Bossert, Klaus D. Jandt
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Publication date: Available online 3 June 2020Source: Microbiological ResearchAuthor(s): Tian Yuan, Zhuang Yihui, Chen Zhujun, Mao Yinhe, Zhang Jin, Lu Renquan, Guo Lin
Source: Microbiological Research - Category: Infectious Diseases Source Type: research
Publication date: Available online 30 May 2020Source: BurnsAuthor(s): Mohammad Hossein Farjah, Mohammad Reza Farahpour
Source: Burns - Category: Dermatology Source Type: research
Mousa, Mohammed A. / Lynch, Edward / Kielbassa, Andrej M.Page 554 - 565Objectives: To assess the relationship between the development of denture-related stomatitis (DRS) and the identification of commonly isolated yeast species, and to evaluate various predisposing factors in Saudi participants wearing new removable dental prostheses.Method and materials: A total of 75 edentulous male participants were recruited, and 64 patients finished the present case-series. All participants received new conventional complete dentures. Colonization of Candida species was assessed, and species were identified by means of the VITEK 2 (bi...
Source: Quintessence International - Category: Dentistry Source Type: research
ConclusionThis study comprehensively assessed biofilm-related microbial strategies that C. albicans uses in vaginal candidiasis and provided experimental evidence to support the important role of biofilm formation in the histopathogenesis of vaginal candidiasis and the recalcitrance of the infection to antifungal treatment.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Invasive fungal infections caused by Candida species affect approximately 700,000 people worldwide resulting in 300,000 deaths annually. Besides Candida albicans, other members of the genus have gained relevance in the last two decades, including C. parapsilosis whose incidence is particularly high amongst low birth weight neonates. To investigate the virulence properties of this pathogen several techniques have been developed for generating knock-out mutants, however, no target locus for knock-in approaches have been published so far. Here we report CpNEUT5L (N5L), an intergenic locus in C. parapsilosis, and introduce an ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract Oral problems are common in patients diagnosed with Eating Disorders (ED) and still require better elucidation. We aimed to analyze the prevalence of oral Candida spp in individuals with ED. The sample of the study was comprised of 30 women with purgative habits and 15 without purgative habits. Samples of the oral cavity were collected by sterile cotton swab rubbed on soft tissues and teeth. Yeasts were isolated on Sabouraud dextrose agar. Yeasts were isolated from the oral cavity of 53% of the patients yielding 75 yeast isolates; of these, 43 were identified by conventional mycological methods: C. paraps...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - Category: Tropical Medicine Authors: Tags: Rev Inst Med Trop Sao Paulo Source Type: research
Publication date: Available online 2 June 2020Source: Journal of EthnopharmacologyAuthor(s): Qiandan Miao, Linjing Zhao, Yuting Wang, Fangjia Hao, Peipei Sun, Peng He, Yumin Liu, Jiashuai Huang, Xijian Liu, Xiaohui Liu, Guoying Deng, Hongsen Li, Lingai Li, Yingao Tang, Lixin Wang, Meiqing Feng, Wei Jia
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
The Journal of Organic ChemistryDOI: 10.1021/acs.joc.0c00402
Source: The Journal of Organic Chemistry - Category: Chemistry Authors: Source Type: research
Abstract Strategies to immobilize the individual enzymes are crucial for enhancing catalytic applicability and require a controlled immobilization process. Herein, protocol for immobilizing Candida rugosa lipase (CRL) onto modified magnetic silica derived from oil palm leaves ash (OPLA) was optimized for the effects of concentration of CRL, immobilization time, and temperature, monitored by titrimetric and spectrometric methods. XRD and TGA-DTG spectrometric observations indicated that OPLA-silica was well coated over magnetite (SiO2-MNPs) and CRLs were uniformly bound by covalent bonds to SiO2-MNPs (CRL/Gl-A-SiO2...
Source: Applied Biochemistry and Biotechnology - Category: Biochemistry Authors: Tags: Appl Biochem Biotechnol Source Type: research
More News: Candida | Genetics | Study