In vitro and in vivo study on the synergistic effect of minocycline and azoles against pathogenic fungi.

In vitro and in vivo study on the synergistic effect of minocycline and azoles against pathogenic fungi. Antimicrob Agents Chemother. 2020 Apr 06;: Authors: Gao L, Sun Y, Yuan M, Li M, Zeng T Abstract In vitro and in vivo interactions of minocycline and azoles including itraconazole, voriconazole, and posaconazole against filamentous pathogenic fungi were investigated. A total of 56 clinical isolates were studied in vitro via broth microdilution checkerboard technique, including 20 strains of Aspergillus fumigatus, 7 strains of A. flavus, 16 strains of Exophiala dermatitidis, 10 strains of Fusarium solani and 3 strain s of F. oxysporum The results revealed that minocycline individually did not exhibit any significant antifungal activity against all tested strains. However, favorable synergy of minocycline with itraconazole, voriconazole, or posaconazole were observed against 34 (61%), 28 (50%), and 38 (69%) isolates, respectively, including azole resistant A. fumigatus and Fusarium spp. with inherently high MICs of azoles. Synergistic combinations resulted in 4 fold to 16-fold reduction of effective MICs of minocycline and azoles. No antagonism was observed. In vivo effect of minocycline-azole combinations were evaluated by survival assay in Galleria mellonella model infected with E. dermatitidis strain BMU00034, F. solani strain FS9, A. fumigatus strain AF293, AFR1 and AFR2 . Minocycline acted synergistically with azoles and significantly increased larv...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research

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ron G Abstract The treatment of invasive and chronic aspergillosis involves triazole drugs. Its intensive use has resulted in the selection of resistant isolates and at present, azole resistance in Aspergillus fumigatus is considered an emerging threat to public health worldwide.The aim of this work is to uncover the molecular mechanism implicated in the azole resistance phenotype of three Aspergillus fumigatus clinical strains isolated from an Argentinian cystic fibrosis patient under long-term triazole treatment.Strain susceptibilities were assessed and CYP51A gene sequences were analyzed. Two of the studied Asp...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Abstract Invasive pulmonary aspergillosis and mucormycosis are life-threatening complications in immunocompromised patients. A rapid diagnosis followed by early antifungal treatment is essential for patient survival. Given the limited spectrum of biomarkers for invasive mold infections, recent studies have proposed radiolabeled siderophores or antibodies as molecular probes to increase the specificity of radiological findings by nuclear imaging modalities. While holding enormous diagnostic potential, most of the currently available molecular probes are tailored to the detection of Aspergillus species and their cos...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
In conclusion, A. niger should be considered as a minority species in the section Nigri The differences in MICs between species for different azoles underline the importance of accurate identification. Significant divergences in the determination of MIC between EUCAST and gradient concentration strips methods require further investigation. PMID: 32312779 [PubMed - as supplied by publisher]
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
This study aims to identify factors that impact survival in 1 of the largest cohorts to date.MethodsPathology records were reviewed for biopsy ‐proven CIFS from 3 tertiary academic institutions from 1995 to 2016. Variables were analyzed using log‐rank survival analysis. Univariate Cox regression was performed at 1 and 12 months.ResultsThirty ‐eight patients were included. Hematologic malignancy and diabetes were the most common underlying diseases (32% each).Aspergillus was the most common fungus (63%). Greater than 75% of the patients had an absolute neutrophil count (ANC)>1000 at the time of diagnosis. Overall s...
Source: International Forum of Allergy and Rhinology - Category: Allergy & Immunology Authors: Tags: ORIGINAL ARTICLE Source Type: research
Invasive aspergillosis (IA) is a life-threatening infection that affects an increasing number of patients undergoing chemotherapy or allo-transplantation, and recent studies have shown that genetic factors contribute to disease susceptibility. In this two-stage, population-based, case-control study, we evaluated whether 7 potentially functional single nucleotide polymorphisms (SNPs) within the ARNT2 and CX3CR1 genes influence the risk of IA in high-risk hematological patients. We genotyped selected SNPs in a cohort of 500 hematological patients (103 of those had been diagnosed with proven or probable IA), and we evaluated ...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Fungal and Parasitic Infections Source Type: research
Combination Therapy with Ibrexafungerp (formerly SCY-078), a First-in-Class Triterpenoid Inhibitor of (1→3)-β-D-Glucan Synthesis, and Isavuconazole for Treatment of Experimental Invasive Pulmonary Aspergillosis. Antimicrob Agents Chemother. 2020 Mar 16;: Authors: Petraitis V, Petraitiene R, Katragkou A, Maung BBW, Naing E, Kavaliauskas P, Barat S, Borroto-Esoda K, Azie N, Angulo D, Walsh TJ Abstract Ibrexafungerp (formerly SCY-078) is a semisynthetic triterpenoid and potent (1→3)-β-D-glucan synthase inhibitor. We investigated the in vitro activity, pharmacokinetics, and in vivo eff...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
AbstractPurpose of ReviewTo describe the clinical features, diagnosis, and treatment of angioinvasive fungal infections.Recent FindingsCandida auris is a recently discovered species with multi- to pan-drug resistance to azoles, amphotericin B, and echinocandins. The SECURE trial showed that isavuconazole was non-inferior to voriconazole for treatment ofAspergillus. Additionally, the VITAL study showed that there was no difference in mortality between those treated with either isavuconazole or amphotericin B. Isavuconazole is now approved by the FDA for the treatment ofAspergillus andMucor.SummaryInvasive fungal infections ...
Source: Current Dermatology Reports - Category: Dermatology Source Type: research
Conference abstracts
Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
Mucormycosis in large cohort of pediatric and adult patients after hematopoietic stem cell transplantation&chemotherapyConference abstracts
Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
In this study, a different endophytic fungus was obtained from a particular medicinal plant, Tarenna asiatica, and was selected for Taxol production. Among 28 screened fungi, only six fungal species produced Taxol in the modified liquid medium. The novel Taxol-producing endophytic fungi were identified through morphological and molecular identification using DNA sequencing and phylogenetic tree construction as Aspergillus oryzae. The structure of Taxol obtained from A. oryzae was identified with the appropriate control using an ultraviolet–visible (UV–Vis) spectrophotometer (235 nm), thin layer chromatography...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
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