“These Aren’t the Strains You’re Looking for”: Recovery Bias of Common Campylobacter jejuni Subtypes in Mixed Cultures

Microbiological surveillance of the food chain plays a critical role in improving our understanding of the distribution and circulation of food-borne pathogens along the farm to fork continuum toward the development of interventions to reduce the burden of illness. The application of molecular subtyping to bacterial isolates collected through surveillance has led to the identification of strains posing the greatest risk to public health. Past evidence suggests that enrichment methods for Campylobacter jejuni, a leading bacterial foodborne pathogen worldwide, may lead to the differential recovery of subtypes, obscuring our ability to infer the composition of a mixed-strain sample and potentially biasing prevalence estimates in surveillance data. To assess the extent of potential selection bias resulting from enrichment-based isolation methods, we compared enrichment and non-enrichment isolation of mixed subtype cultures of C. jejuni, followed by subtype-specific enumeration using both colony plate-counts and digital droplet PCR. Results differed from the null hypothesis that similar proportions of C. jejuni subtypes are recovered from both methods. Our results also indicated a significant effect of subtype prevalence on isolation frequency post-recovery, with the recovery of more common subtypes being consistently favored. This bias was exacerbated when an enrichment step was included in the isolation procedure. Taken together, our results emphasize the importance of selecting...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Publication date: Available online 25 May 2020Source: Research in Veterinary ScienceAuthor(s): M.V. Zbrun, E. Rossler, A. Romero-Scharpen, L.P. Soto, A. Berisvil, J.A. Zimmermann, M.L. Fusari, M.L. Signorini, L.S. Frizzo
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 24 May 2020Source: Veterinary MicrobiologyAuthor(s): Khaled Taha-abdelaziz, Jake Astill, Bahram Shojadoost, Silvia Borrelli, Mario A Monteiro, Shayan Sharif
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Publication date: Available online 24 May 2020Source: Veterinary MicrobiologyAuthor(s): Dejun Liu, Dawei Yang, Xiao Liu, Xing Li, Andrea Feßler, Zhangqi Shen, Jianzhong Shen, Stefan Schwarz, Yang Wang
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
This study provides an important baseline census of the standing pangenomic variation and drug resistance to aid the development of a statewide database for epidemiological studies and clinical decision making. Continued genomic surveillance will be necessary to accurately assess how the population of C. jejuni changes over the long term.
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Epidemiology Source Type: research
Campylobacter jejuni monitors intestinal metabolites produced by the host and microbiota to initiate intestinal colonization of avian and animal hosts for commensalism and infection of humans for diarrheal disease. We previously discovered that C. jejuni has the capacity to spatially discern different intestinal regions by sensing lactate and the short-chain...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: Biological Sciences Source Type: research
Sequelae of Campylobacter and NTS infections are rare but associated with increased NHS costs. Prior prescription of PPI may be a modifiable risk factor. Incidence of sequelae, healthcare resource use and costs are essential parameters for future burden of disease studies.
Source: Journal of Infection - Category: Infectious Diseases Authors: Source Type: research
Modern agriculture has dramatically changed the distribution of animal species on Earth. Changes to host ecology have a major impact on the microbiota, potentially increasing the risk of zoonotic pathogens being transmitted to humans, but the impact of intensive livestock production on host-associated bacteria has rarely been studied. Here, we...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: Biological Sciences Source Type: research
Lipooligosaccharide (LOS) is an integral component of the Campylobacter cell membrane with a structure of core oligosaccharides forming inner and outer core regions and a lipid A moiety. The gene content of the LOS core biosynthesis cluster exhibits extensive sequence variation, which leads to the production of variable cell surface LOS structures in Campylobacter. Some LOS outer core molecules in Campylobacter jejuni are molecular mimics of host structures (such as neuronal gangliosides) and are thought to trigger neuronal disorders (particularly Guillain–Barré syndrome and Miller Fisher syndrome) in humans. ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 16 May 2020Source: Preventive Veterinary MedicineAuthor(s): Md. Mehedi Hasan, Sudipta Talukder, Amit Kumar Mandal, Syeda Tanjina Tasmim, Mst. Sonia Parvin, Md. Yamin Ali, Mahmudul Hasan Sikder, Md. Taohidul Islam
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
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Source: Critical Reviews in Microbiology - Category: Microbiology Authors: Tags: Crit Rev Microbiol Source Type: research
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