Metabolic Infrastructure of Pregnant Women with Methylenetetrahydrofolate Reductase Polymorphisms: A Metabolomic Analysis.

Metabolic Infrastructure of Pregnant Women with Methylenetetrahydrofolate Reductase Polymorphisms: A Metabolomic Analysis. Biomed Chromatogr. 2020 Apr 08;:e4842 Authors: Recber T, Orgul G, Aydın E, Tanacan A, Nemutlu E, Kır S, Beksac MS Abstract The aim of this study is to demonstrate altered metabolic infrastructure of pregnant women with methylenetetrahydrofolate reductase (MTHFR) polymorphisms at first-trimester and during delivery. Eight singleton pregnant women with MTHFR polymorphisms were compared with 10 normal pregnant women. Maternal blood samples were withdrawn twice during their pregnancies (between the 11th and 14th gestational weeks and during delivery). Metabolomic analysis was performed by gas chromatography-mass spectrometry (GC-MS). The GC-MS-based metabolomics profile helped identify 95 metabolites in the plasma samples. In the MTHFR group, 1-monohexadecanoylglycerol, pyrophosphate, benzoin, and linoleic acid were found to be significantly decreased (p ˂ 0.05 for all), whereas glyceric acid, L-tryptophan, L-alanine, L-proline, norvaline, L-threonine, and myo-inositol were significantly increased (p ˂ 0.01 for the first 2 metabolites, p ˂ 0.05 for the others) at 11-14 gestational weeks. Conversely, benzoin, 1-monohexadecanoylglycerol, pyruvic acid, L-proline, phosphoric acid, epsilon-caprolactam, and pipecolic acid were significantly decreased in the MTHFR group, whereas metabolites such as hexadecanoic acid an...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research