Citral Induced Apoptosis through Modulation of Key Genes Involved in Fatty Acid Biosynthesis in Human Prostate Cancer Cells: In Silico and In Vitro Study.

Citral Induced Apoptosis through Modulation of Key Genes Involved in Fatty Acid Biosynthesis in Human Prostate Cancer Cells: In Silico and In Vitro Study. Biomed Res Int. 2020;2020:6040727 Authors: Balusamy SR, Perumalsamy H, Veerappan K, Huq MA, Rajeshkumar S, Lakshmi T, Kim YJ Abstract The isomers of citral (cis-citral and trans-citral) were isolated from the Cymbopogon citratus (DC.) Stapf oil demonstrates many therapeutic properties including anticancer properties. However, the effects of citral on suppressing human prostate cancer and its underlying molecular mechanism have yet to be elucidated. The citral was isolated from lemongrass oil using various spectroscopic analyses, such as electron ionized mass spectrometry (EI-MS) and nuclear magnetic resonance (NMR) spectroscopy respectively. We carried out 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to evaluate the cell viability of citral in prostate cancer cells (PC-3 and PC3M). Furthermore, to confirm that PC3 undergoes apoptosis by inhibiting lipogenesis, we used several detection methods including flow cytometry, DNA fragmentation, Hoechst staining, PI staining, oil staining, qPCR, and Western blotting. Citral impaired the clonogenic property of the cancer cells and altered the morphology of cancer cells. Molecular interaction studies and the PASS biological program predicted that citral isomers tend to interact with proteins involved in lipogenesis and the apoptosis ...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research

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Authors: Lin M, Bu C, He Q, Gu J, Wang H, Feng N, Jiang SW Abstract TAZ (transcriptional coactivator with PDZ‑binding motif), which is also known as WW domain‑containing transcription regulator 1 (WWTR1), a downstream effector of the Hippo pathway, has been reported to regulate cancer cell proliferation, migration and apoptosis by acting as a transcriptional coactivator. However, the function of TAZ in prostate cancer cells has not been investigated. In the present study, TAZ expression in prostate cancer (PCa) and benign prostatic hyperplasia tissues, PCa cell lines, and normal prostate epithelial cells was de...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Final data analysis of SPARTAN trial demonstrates large benefit in overall survival when compared with ADT plus placebo.
Source: CancerNetwork - Category: Cancer & Oncology Authors: Source Type: news
Authors: Moussa M, Papatsoris A, Abou Chakra M, Sryropoulou D, Dellis A Abstract INTRODUCTION: Metastatic castration-resistant prostate cancer (CRPC) is a potentially symptomatic disease with an eventual lethal outcome. Novel pharmaceutical agents are continuously studied with encouraging results in CRPC. AREAS COVERED: In this perspective, the authors present established and promising pharmacotherapeutic strategies for the management of CRPC; both with and without metastases. Apart from the different treatment strategies, the authors present the relevant sequence of treatment through disease progression. E...
Source: Expert Opinion on Pharmacotherapy - Category: Drugs & Pharmacology Tags: Expert Opin Pharmacother Source Type: research
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Source: International Urology and Nephrology - Category: Urology & Nephrology Source Type: research
Publication date: Available online 30 May 2020Source: Annals of Medicine and SurgeryAuthor(s): Francesco Ziglioli, Marco Baciarello, Giada Maspero, Valentina Bellini, Tommaso Bocchialini, Domenico Cavalieri, Elena Giovanna Bignami, Umberto Maestroni
Source: Annals of Medicine and Surgery - Category: General Medicine Source Type: research
Abstract Identification and clinical translation of routinely tested biomarkers require a complex and multistep workflow. Here, we described a confirmatory process estimating the utility of previously identified candidate tissue miRNAs for diagnosis of prostate cancer (PCa). RNA was isolated from formalin-fixed paraffin-embedded (FFPE) prostate tissue surgically resected from 44 patients with PCa and 24 patients with benign prostate hyperplasia (BPH). Of the 92 RNA samples obtained, 68 represented 42 malignant (PCa) areas and 26 represented nonmalignant (PCa 0%) areas of the prostate tissue sections. The levels of...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
The results suggested the potential for this agent to become a new standard for T-suppression in this patient population.
Source: CancerNetwork - Category: Cancer & Oncology Authors: Source Type: news
ConclusionsThe increase in a number of genes associated with ribosome biogenesis, translation, and mTOR signaling may be important to evaluate prognosis and determine treatment approach for prostate cancer (PCa). A better understanding of the molecular pathways associated with CSCs may be promising to develop targeted therapies to prolong survival in PCa.
Source: Clinical and Translational Oncology - Category: Cancer & Oncology Source Type: research
DiscussionThe RCI may be used to aid medical decision making in older Spanish men with PC, especially in those with a high RCI 5 and higher.
Source: Clinical and Translational Oncology - Category: Cancer & Oncology Source Type: research
An amendment to this paper has been published and can be accessed via the original article.
Source: Molecular Cancer - Category: Cancer & Oncology Authors: Tags: Correction Source Type: research
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