Increased production of periplasmic proteins in Escherichia coli by directed evolution of the translation initiation region

Recombinant proteins are often engineered with an N-terminal signal peptide, which facilitates their secretion to the oxidising environment of the periplasm (gram-negative bacteria) or the culture supernatant ...

Source: Microbial Cell Factories - April 7, 2020 Category: Microbiology Authors: Kiavash Mirzadeh, Patrick J. Shilling, Rageia Elfageih, Alister J. Cumming, Huanhuan L. Cui, Maja Rennig, Morten H. H. N ørholm and Daniel O. Daley Tags: Research Source Type: research