Enzymatic synthesis of biolubricants from by-product of soybean oil processing catalyzed by different biocatalysts of Candida rugosa lipase

Publication date: Available online 7 April 2020Source: Catalysis TodayAuthor(s): Keysson V. Fernandes, Elisa D.C. Cavalcanti, Eliane P. Cipolatti, Erika C.G. Aguieiras, Martina C.C. Pinto, Fernanda A. Tavares, Priscila R. da Silva, Roberto Fernandez-Lafuente, Sara Arana-Peña, José Carlos Pinto, Charles L.B. Assunção, José André C. da Silva, Denise M.G. Freire
Source: Catalysis Today - Category: Chemistry Source Type: research

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Centromeres ofCandida albicans form on unique and different DNA sequences but a closely related species,Candida tropicalis, possesses homogenized inverted repeat (HIR)-associated centromeres. To investigate the mechanism of centromere type transition, we improved the fragmented genome assembly and constructed a chromosome-level genome assembly ofC. tropicalis by employing PacBio sequencing, chromosome conformation capture sequencing (3C-seq), chromoblot, and genetic analysis of engineered aneuploid strains. Further, we analyzed the 3D genome organization using 3C-seq data, which revealed spatial proximity among the centrom...
Source: eLife - Category: Biomedical Science Tags: Chromosomes and Gene Expression Genetics and Genomics Source Type: research
Surgical Infections, Ahead of Print.
Source: Surgical Infections - Category: Surgery Authors: Source Type: research
c F, Nováková L Abstract The new ultra-high performance liquid chromatography method with tandem mass spectrometry detection (UHPLC-MS/MS) has been optimized to allow fast, selective, and high-throughput analysis of two Candida albicans quorum sensing molecules (QSM), farnesol and tyrosol. The problem of the presence of the interference in the samples and system was successfully solved by careful optimization of chromatographic conditions. Charged hybrid stationary phase modified with pentafluorophenyl group and optimized gradient elution provided adequate separation selectivity and peak shapes. The ...
Source: Analytical and Bioanalytical Chemistry - Category: Chemistry Authors: Tags: Anal Bioanal Chem Source Type: research
We report here on a 31 years old female patient suffering from severe mucosal aphthous mucositis and ulcers and recurrent herpes simplex for decades. We found a previously unknown heterozygous sequence variant in STAT1 (c.1219C>G; L407V) affecting the DNA-binding domain of the protein in the patient and her 4 years old daughter. We found this mutation gain-of-function (GOF) by using immunoblot and luciferase assays. We detected low proportion of IL-17A-producing CD4+ T cell lymphocytes by using intracellular staining and flow cytometry. Candida-induced secretion of IL-17A and IL-22 by mononuclear cells from the patient ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 26 May 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Anderson da Silva, Hélio Nobre, Leticia Sampaio, Bruna do Nascimento, Cecilia da Silva, João Batista de Andrade Neto, Ángeles Manresa, Aurora Pinazo, Bruno Cavalcanti, Manoel Odorico de Moraes, Iñaki Ruiz-Trillo, Meritxell Antó, Carmen Morán, Lourdes Pérez
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Publication date: Available online 23 May 2020Source: Urology Case ReportsAuthor(s): Pravin Viswambaram, Jeanie Misko, Matthew Rawlins, Sarah Clark, John Dyer, Dickon Hayne
Source: Urology Case Reports - Category: Urology & Nephrology Source Type: research
Candida diddensiae, a yeast found in olive oil, is considered non-pathogenic to humans. Here, we describe the first case of fungemia caused by C. diddensiae in a hospitalized patient with underlying diseases.
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Case report Source Type: research
We report here that isolates of the pathogenic yeast species Candida parapsilosis show within-species polymorphism for the location of centromeres on two of its eight chromosomes. Its old centromeres have an inverted-repeat (IR) structure, whereas its new centromeres have no obvious structural features but are located within 30 kb of the old site. Centromeres can therefore move naturally from one chromosomal site to another, apparently spontaneously and in the absence of any significant changes in DNA sequence. Our observations are consistent with a model in which all centromeres are genetically determined, such as by the ...
Source: Genome Research - Category: Genetics & Stem Cells Authors: Tags: RESEARCH Source Type: research
Small molecule inhibitors targeting sterol 14α-demethylase (CYP51): synthesis, molecular modelling and evaluation against Candida albicans. ChemMedChem. 2020 May 27;: Authors: Simons C, Binjubair F, Warrilow AG, Puri K, Braidley PJ, Tatar E, Kelly SL, Kelly DE, Parker J Abstract Fungal infections are a global issue affecting over 150 million people worldwide annually with 750,000 of these caused by invasive Candida infections. Azole drugs are the frontline treatment against fungal infections however resistance to current azole antifungals in C. albicans poses a threat to public health. Two serie...
Source: ChemMedChem - Category: Chemistry Authors: Tags: ChemMedChem Source Type: research
Publication date: September 2020Source: Materials Today Communications, Volume 24Author(s): Jeferson Almeida Dias, Alexandre José Gualdi, Leonardo José Dalla Costa, Rosario Elida Suman Bretas, Ana Candida Martins Rodrigues, Márcio Raymundo Morelli
Source: Materials Today Communications - Category: Materials Science Source Type: research
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