miR-149 rs2292832 C allele enhances the cytotoxic effect of temozolomide against glioma cells

This study aims to assess the impact of miR-149 rs2292832 C/T polymorphism and miR-149-5p expressions in cytotoxic effect of temozolomide against glioma cells. A total of 137 cases of glioma patients and 21 healthy cases were enrolled in this study for clinical research. We found that miR-149-5p was significantly downregulated in glioma cell lines and in blood leukocyte of glioma patients. Furthermore, miR-149 rs2292832 C/T polymorphism was significantly associated with glioma prognosis and temozolomide resistance. Subsequently, the glioma cell lines stable transfected with common miR-149 expression construct (miR-149-T) and the variant miR-149 expression construct (miR-149-C) were used to determine the regulatory effect of miR-149 rs2292832 C on glioma cells progression. Data revealed that miR-149 rs2292832 C allele could enhance the miR-149-5p expressions, and therefore, prevent the proliferation of glioma cells and increase the cytotoxicity of temozolomide against glioma cells. These functions of miR-149-C were demonstrated to be triggered by CDK6/SOX2 pathway inhibition. The above results demonstrated that miR-149 rs2292832 C/T polymorphism was a potential prognostic biomarker for glioma development by regulating miR-149/CDK6 axis.
Source: NeuroReport - Category: Neurology Tags: Integrative Systems Source Type: research

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Publication date: 2020Source: International Review of Neurobiology, Volume 151Author(s): Sergei Zaitsev, Hari Shanker Sharma, Aruna Sharma, Igor Manzhulo, Alexander Polevshchikov, Igor Kudriavtsev, Yuri Khotimchenko, Oleg Pak, Andrey Bryukhovetskiy, Igor Bryukhovetskiy
Source: International Review of Neurobiology - Category: Neuroscience Source Type: research
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Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
Temozolomide (TMZ) is considered a standard chemotherapeutic agent for glioblastoma (GBM). Characterizing the biological molecules and signaling pathways involved in TMZ sensitivity would be helpful for selecting therapeutic schemes and evaluating prognosis for GBM. Thus, in the present study, we selected 34 glioma cell lines paired with specific IC50 values of TMZ obtained from CancerRxGene and RNA-seq data downloaded from the Cancer Cell Line Encyclopedia to identify genes related to TMZ sensitivity. The results showed that 1,373 genes were related to the response of GBM cells to TMZ. Biological function analysis indicat...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
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Source: Journal of Neurochemistry - Category: Neuroscience Authors: Tags: ORIGINAL ARTICLE Source Type: research
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Source: Journal of Neuro-Oncology - Category: Cancer & Oncology Source Type: research
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Source: Clinical Cancer Research - Category: Cancer & Oncology Authors: Tags: Clin Cancer Res Source Type: research
Although Mediterranean diet (MD) was associated with a lower risk of mortality and cancer, no data are available investigating the association between adherence to the MD and risk of glioma.
Source: Clinical Nutrition - Category: Nutrition Authors: Tags: Original article Source Type: research
The T2-FLAIR mismatch sign is an imaging finding highly suggestive of isocitrate dehydrogenase mutated (IDH-mut) 1p19q non-codeleted (non-codel) gliomas (astrocytomas). In previous studies, it has shown excellent...
Source: BMC Cancer - Category: Cancer & Oncology Authors: Tags: Research article Source Type: research
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Source: PLoS Computational Biology - Category: Biology Authors: Source Type: research
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Source: Cellular and Molecular Neurobiology - Category: Cytology Authors: Tags: Cell Mol Neurobiol Source Type: research
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