Synthesis, characterization, DNA binding and cytotoxicity studies of two novel Cu(II)-2-(2'-pyridyl) quinoxaline complexes.

Synthesis, characterization, DNA binding and cytotoxicity studies of two novel Cu(II)-2-(2'-pyridyl) quinoxaline complexes. J Inorg Biochem. 2020 Mar 23;208:111077 Authors: Lioli E, Psycharis V, Raptopoulou CP, Efthimiadou EK, Mitsopoulou CA Abstract Two novel copper (II) complexes, namely [Cu(2,2'-pq)(NO3)](NO3) (1) and [Cu(2,2'-pq)2(NO3)](NO3)·6H2O(2) where 2,2'-pq is 2-(2'-pyridyl quinoxaline) were synthesized and characterized by various spectral methods. Complex 2 characterized by single crystal X-ray diffraction showed a distorted trigonal bipyramidal geometry and crystallized together with an hexamer, (H2O)6 cluster, with R66(12) topology and chair conformation. The interaction of Calf Thymus DNA (CT-DNA) with 1 and 2 was investigated by UV-Visible absorption spectra, Viscosity, Cyclic Voltammetry, Fluorescence and Circular Dichroism (CD), indicating that both complexes can bind to DNA both by means of intercalation and groove binding. Their DNA interaction mode depends on their concentrations and the differences between their structures. Cleavage experiments were performed by agarose gel electrophoresis using pBR322 DNA both in dark and after illumination. Furthermore, the cytotoxicity of both complexes was evaluated against MCF-7 and healthy cells (HEK-293) by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays; moreover their cell uptake against MCF-7 tumor cells and HEK-293 normal cells was revealed ...
Source: Journal of Inorganic Biochemistry - Category: Biochemistry Authors: Tags: J Inorg Biochem Source Type: research