Sirt1-ROS-TRAF6 Signaling-Induced Pyroptosis Contributes to Early Injury in Ischemic Mice

In this study, we evaluated the inflammatory reactions induced by acute ischemia and found that pyroptosis occurred after acute ischemia bothin vivo andin vitro, as determined by interleukin-1 β, apoptosis-associated speck-like protein, and caspase-1. The early inflammation resulted in irreversible ischemic injury, indicating that it deserves thorough investigation. Meanwhile, acute ischemia decreased the Sirtuin 1 (Sirt1) protein levels, and increased the TRAF6 (TNF receptor associated factor 6) protein and reactive oxygen species (ROS) levels. In further exploration, both Sirt1 suppression and TRAF6 activation were found to contribute to this pyroptosis. Reduced Sirt1 levels were responsible for the production of ROS and increased TRAF6 protein levels after ischemic exposure. Mor eover, N-acetyl-L-cysteine, an ROS scavenger, suppressed the TRAF6 accumulation induced by oxygen-glucose deprivationvia suppression of ROS bursts. These phenomena indicate that Sirt1 is upstream of ROS, and ROS bursts result in increased TRAF6 levels. Further, the activation of Sirt1 during the period of ischemia reduced ischemia-induced injury after 72  h of reperfusion in mice with middle cerebral artery occlusion. In sum, these results indicate that pyroptosis-dependent machinery contributes to the neural injury during acute ischemiavia the Sirt1-ROS-TRAF6 signaling pathway. We propose that inflammatory reactions occur soon after oxidative stress and are detrimental to neuronal surviv...
Source: Neuroscience Bulletin - Category: Neuroscience Source Type: research

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In conclusion, the findings of the present study suggested that miR‑340‑5p may protect PC12 cells against OGD/R through targeting Neurod4, which could provide important implications for the treatment of ischemia‑reperfusion injury based on miR‑340‑5p expression levels in vivo. PMID: 32468054 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Authors: Susmitha K, Chandrasekhar V, Kiran G, Divya A, Rani GS, Sarangapani M Abstract Stroke is becoming a main cause of early death and disability in developing countries like India, and it is mostly enhanced by increased predominance of major risk factors. A detailed knowledge about the nature and magnitude of the stroke cases in this particular area is not only important for acute treatment but also it helps to prevent hospital admissions due to reoccurring stroke. The present study was conducted in the Department of Stroke at MGM Hospital, Warangal, India, to study the patterns of stroke admissions. All the c...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
Contributors : Yejie Shi ; Jun ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSenescence-associated alterations in microglia may have profound impact on cerebral homeostasis and stroke outcomes. However, the lack of a transcriptome-wide comparison between young and aged microglia in the context of ischemia limits our understanding of aging-related mechanisms. Herein, we performed bulk RNA sequencing analysis of microglia purified from cerebral hemispheres of young adult (10-week-old) and aged (18-month-old) mice 5 days after distal middle cerebral artery occlusion or sham operati...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Strokes remain a leading cause of morbidity and mortality in patients with ventricular assist devices (VADs). Varying study populations, event definitions, and reporting methods make direct comparison of neurologic event risk across clinical trials and registries challenging. We aim to highlight important differences among major VAD studies and standardize rates of neurologic events to facilitate a comprehensive and objective comparison. We systematically identified and analyzed key clinical trials and registries evaluating the HeartMate II (HMII), HeartMate 3 (HM3), and HVAD devices. Reported neurologic events were nonexc...
Source: ASAIO Journal - Category: Medical Devices Tags: Adult Circulatory Support Source Type: research
The role of metabolically unhealthy (MU) overweight in influencing stroke prognosis has not been fully assessed and conclusive data about the impact of sex are lacking. Aim of this study was to evaluate the possible correlations among MU overweight, sex and ischemic stroke severity and outcome.
Source: Journal of the Neurological Sciences - Category: Neurology Authors: Source Type: research
AbstractStroke has a high incidence rate and often leads to permanent disability, particularly if it is not treated promptly. However, no blood biomarkers for early diagnosis are available to date. Therefore, we sought to detect stroke-specific blood biomarkers by identifying proteins associated with the underlying coagulation mechanism, which accounts for more than 80% of all stroke cases. Protein profiling was performed using blood samples from 16 healthy controls and 18 patients who suffered a stroke as the discovery set. We identified upregulated proteins (>  1.5-fold change andp value
Source: Journal of Molecular Neuroscience - Category: Neuroscience Source Type: research
Condition:   Stress Hyperglycemia and Stroke Intervention:   Other: standard protocol for stroke patients Sponsor:   Ain Shams University Completed
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Conditions:   Stroke, Ischemic;   Coronary Artery Disease;   Angina Pectoris, Stable;   Diabetic Peripheral Neuropathy Interventions:   Drug: Buqitongluo Granule;   Drug: Buqitongluo Granule Placebo;   Other: Standard care Sponsors:   Dongzhimen Hospital, Beijing;   The Affiliated Hospital of Changchun University of Chinese Medicine;   The Affiliated Hospital of Shandong University of Chinese Medicine;   The Affiliated Hospital of Chengdu University of Chinese Medicine;   The Second Af...
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Publication date: Available online 28 May 2020Source: American Journal of Kidney DiseasesAuthor(s): James B. Wetmore, Charles A. Herzog, Anne Sexter, David T. Gilbertson, Jiannong Liu, Scott E. Kasner
Source: American Journal of Kidney Diseases - Category: Urology & Nephrology Source Type: research
Conditions:   Stroke, Ischemic;   Coronary Artery Disease;   Angina Pectoris, Stable;   Diabetic Peripheral Neuropathy Interventions:   Drug: Buqitongluo Granule;   Drug: Buqitongluo Granule Placebo;   Other: Standard care Sponsors:   Dongzhimen Hospital, Beijing;   The Affiliated Hospital of Changchun University of Chinese Medicine;   The Affiliated Hospital of Shandong University of Chinese Medicine;   The Affiliated Hospital of Chengdu University of Chinese Medicine;   The Second Af...
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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