Rapid and sensitive detection of VBNC Escherichia coli O157: H7 in beef by PMAxx and real-time LAMP

Publication date: Available online 4 April 2020Source: Food ControlAuthor(s): Xinrui Lv, Li Wang, Jingfeng Zhang, Haiyan Zeng, Xun Chen, Lei Shi, Hualing Cui, Xiaoxin He, Lichao Zhao
Source: Food Control - Category: Food Science Source Type: research

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Authors: Sharifdini M, Ghanbarzadeh L, Barikani A, Saraei M Abstract Background: Intestinal parasitic infections (IPIs) are among the most important etiologies of gastrointestinal disorders in developing countries. The present study was performed to determine the prevalence of IPIs in rural inhabitants of Fouman, northern Iran. Methods: Overall, 31 villages were randomly selected during 2015-2016. Stool samples were collected from 1500 inhabitants aged 2-87. The samples were examined by direct wet smear, formalin ethyl-acetate concentration and agar plate culture. Trichrome staining and modified acid-fast stain...
Source: Iranian Journal of Parasitology - Category: Parasitology Tags: Iran J Parasitol Source Type: research
Publication date: Available online 3 June 2020Source: Process BiochemistryAuthor(s): Ying Zhang, Liangkun Long, Shaojun Ding
Source: Process Biochemistry - Category: Biochemistry Source Type: research
The present study aims to evaluate the effects of different early weaning paradigms, which supplied with extra alfalfa hay, or starter feeding, or both alfalfa hay and starter feeding, along with the milk replacer, on the gastrointestinal microbial community, growth, and immune performance of yak calves. Twenty 30-day-old male yak calves were randomly assigned to four groups, including the control (CON), alfalfa hay (A), starter feeding (S), and starter plus alfalfa hay (SA) groups. The gastrointestinal microbial colonization, the gastrointestinal development and function, and the growth and immune performance of all the y...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Catechol 1,2 dioxygenases (C12DOs) have been studied for its ability to cleavage the benzene ring of catechol, the main intermediate in the degradation of aromatic compounds derived from aerobic degradation of hydrocarbons. Here we report the genome sequence of the marine bacterium Pseudomonas stutzeri GOM2, isolated from the southwestern Gulf of Mexico, and the biochemical characterization of its C12DO (PsC12DO). The catA gene, encoding PsC12DO of 312 amino acid residues, was cloned and expressed in Escherichia coli. Many C12DOs have been described as dimeric enzymes including those present in Pseudomonas species. The pur...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study focused on the use of recombinant porcine IFNs, produced by Escherichia coli, to inhibit the replication of ASFV. The activity of IFN against ASFV was detected using primary alveolar macrophages at different doses through immunofluorescence assays and quantitative real-time PCR. We found that both 1000 and 100 U/mL doses significantly inhibited the replication of ASFV. Meanwhile, we found that IFNs could significantly trigger the production of a variety of IFN-induced genes (IFIT1, IFITM3, Mx-1, OASL, ISG15, PKR, GBP1, Viperin, BST2, IRF-1, and CXCL10) and MHC molecules, which play key roles in resistance to vir...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Following infection with certain strains of Shiga toxin-producing Escherichia coli (STEC), particularly enterohemorrhagic ones, patients are at elevated risk for developing life-threatening extraintestinal complications, such as acute renal failure. Hence, these bacteria represent a public health concern in both developed and developing countries. Shiga toxins (Stxs) expressed by STEC are highly cytotoxic class II ribosome-inactivating proteins and primary virulence factors responsible for major clinical signs of Stx-mediated pathogenesis, including bloody diarrhea, hemolytic uremic syndrome (HUS), and neurological complic...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Publication date: Available online 1 June 2020Source: Microbial PathogenesisAuthor(s): Weiyu Wang, Menglu Cai, Junying Hu, Zecai Zhang, Xu Wang, Xiaoran Chang, Fan Zhang, Changming Guo, Xinping Wang
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
The well-known fact that avian pathogenic Escherichia coli (APEC) is harder to prevent due to its numerous serogroups has promoted the development of biological immunostimulatory materials as new vaccine candidat...
Source: Microbial Cell Factories - Category: Microbiology Authors: Tags: Research Source Type: research
Publication date: Available online 2 June 2020Source: Food ChemistryAuthor(s): Zonghan Wang, Xiaolin Yao, Yongzhi Zhang, Rong Wang, Yanwei Ji, Jing Sun, Daohong Zhang, Jianlong Wang
Source: Food Chemistry - Category: Food Science Source Type: research
We report herein that EspM4VN was stable from 10°C to 50°C and pH 4 to 10 but deactivated at 70°C and pH 3 and 12. This phage formed clear plaques only on Enterobacter sp. M4 among tested bacterial strains. A one-step growth curve showed that the latent phase was 20 min, rise period was 10 min, and an average of 122 phage particles were released from each absorbed cell. We found the phage’s genome size was 160,766 bp and that it annotated 219 open reading frames. The genome organization of EspM4VN has high similarity with the Salmonella phage SKML-39; Dickeya phages Coodle, PP35, JA15, and Limestone; and ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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