Extracellular vesicles containing the transferrin receptor as nanocarriers of apotransferrin
AbstractPrevious work by our group has shown the pro ‐differentiating effects of apotransferrin (aTf) on oligodendroglial cellsin vivo andin vitro. Further studies showed the remyelinating effect of aTf in animal demyelination models such as hypoxia/ischemia, where the intranasal administration of human aTf provided brain neuroprotection and reduced white matter damage, neuronal loss and astrogliosis in different brain regions.These data led us to search for a less invasive and controlled technique to deliver aTf to the CNS. To such end, we isolated extracellular vesicles (EVs) from human and mouse plasma and different neuron and glia conditioned media and characterized them based on their quality, quantity, identity and structural integrity by Western blot, dynamic light scattering and scanning electron microscopy. All sources yielded highly pure vesicles whose size and structures were in keeping with previous literary evidence. Given that, remarkably, EVs from all sources analyzed contained Tf receptor 1 (TfR1) in their composition, we employed two passive cargo ‐loading strategies which rendered successful EV loading with aTf, specifically through binding to TfR1.These results unveil EVs as potential nanovehicles of aTf to be delivered into the CNS parenchyma, and pave the way for further studies into their possible clinical application in the treatment of demyelinating diseases.
Authors: Miceli A, Raffa S, Donegani MI, Cossu V, Morbelli S, Marini C, Sambuceti G, Bauckneht M Abstract Sarcoidosis is a systemic inflammatory disease of unknown etiology, pathologically characterized by non-caseating granulomas involving several organs and tissues. This pathological process can eventually affect the heart during his course leading to fibrosis associated with systolic dysfunction, conduction disturbance, and even sudden cardiac death. Due to this prognostic impact, diagnosis is crucial to optimize clinical management. The low sensitivity of endomyocardial biopsy and its invasive nature prevents i...
Conclusions. LDL-C is the lipid fraction strongest associated with younger age of presentation of first MI. These results support the importance of controlling and treating LDL-C in prevention of premature MI. PMID: 32483990 [PubMed - as supplied by publisher]
Combination of Fluorine-18 Fluorodeoxyglucose Positron-Emission Tomography/Computed Tomography (¹⁸F-FDG PET/CT) and Tumor Markers to Diagnose Lymph Node Metastasis in Non-Small Cell Lung Cancer (NSCLC): A Retrospective and Prospective Study. Med Sci Monit. 2020 Jun 02;26:e922675 Authors: Zhai X, Guo Y, Qian X Abstract BACKGROUND The early diagnosis of lymph node (LN) metastasis is crucial for patients with non-small cell lung cancer (NSCLC). However, the diagnosis of LN metastasis mainly dependent on ¹⁸F-FDG PET/CT (fluorine-18 fluorodeoxyglucose positron-emission tomography/computed tomo...
The Journal of Prosthetic DentistryAvailable online 27 May 2020https://doi.org/10.1016/j.prosdent.2020.02.034Get rights and contentAbstractStatement of problemThe introduction of intraoral scanners has increased the use of digital technology in dental procedures. However, data on the accuracy of abutment scans according to the distance between the adjacent tooth and the abutment are lacking.PurposeThe purpose of this in vitro study was to compare the 3D accuracy of abutment scans according to the distance between the adjacent tooth and the abutment.Material and methodsA right maxillary first molar was removed and scan...
Authors: Vijayvergiya R, Kanabar K, Kaushal S, Lal A, Krishnan S Abstract A 42-year-old female with end-stage chronic kidney disease, who was on maintenance hemodialysis for the last 3 years, presented with facial and upper-limb swelling of 2-month duration. A computed tomography (CT) scan confirmed significant stenosis of the superior vena cava (SVC). Following discussion in a multidisciplinary meeting, it was proposed to perform a repeat endovascular intervention to relieve the SVC obstruction and manage the migrated stent. PMID: 32479419 [PubMed - in process]
Conclusions: Research evaluating occupational therapy interventions aimed at supporting homeless individuals as they transition to housing is in an early stage of development.Significance: Implications for research and practice are discussed. PMID: 32476575 [PubMed - as supplied by publisher]
Conclusion: CT-guided pudendal nerve block at the sacrospinous ligament and the Alcock's canal was effective in improving premature ejaculation. Therefore, pudendal nerve entrapment may be a curable cause of sensory premature ejaculation. PMID: 32476566 [PubMed - as supplied by publisher]
Conclusions: A distinct temporal hump in mean birthweight among Norwegian term births had no obvious explanations. Furthermore, these fluctuations in birthweight were not associated indirectly with adverse outcomes in measures of infant health.
In conclusion, we suggest that in the presence of intolerance or resistance to colchicine, interleukin (IL)-1 inhibition could suppress peritoneal inflammation and prevent MSTs. PMID: 32477360 [PubMed - in process]
[New Zimbabwe] President Emmerson Mnangagwa's son, Collins has denied any links with a company said to have pocketed nearly US$1 million after being contracted to supply government with grossly inflated coronavirus fighting equipment.