Recombinant protein production and purification of SiiD, SiiE and SiiF - Components of the SPI4-encoded type I secretion system from Salmonella Typhimurium

Publication date: Available online 3 April 2020Source: Protein Expression and PurificationAuthor(s): Stefan Klingl, Sina Kordes, Benedikt Schmid, Roman G. Gerlach, Michael Hensel, Yves A. Muller
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research

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Publication date: Available online 9 July 2020Source: LWTAuthor(s): Seung-Hae Gwak, Jin-Hee Kim, Se-Wook Oh
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
vic Zizovic Supercritical fluid extraction as an environmentally friendly technology was applied to isolate biologically active extracts from celery and parsley fruits for potential applications in the food industry. The extractions were performed under mild temperature conditions of 39.85 °C and at pressures of 10 and 30 MPa. The extracts were analyzed regarding their chemical composition, antibacterial activity, and cytotoxic effect. Sedanolide was the dominant component of the celery fruit extracts, comprising more than 70% of the obtained fraction, while the content of apiole in the parsley fruit SC CO2 ...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
In this study, a detailed evaluation of BAFASAL® preparation was performed. BAFASAL® is a bacteriophage cocktail that reduces Salmonella in poultry farming. In vivo acute and sub-chronic toxicity studies on rats and tolerance study on targeted animals (chicken broiler) conducted according to GLP and OECD guidelines did not reveal any signs of toxicity, which could be associated with BAFASAL® administration. In addition, no evidences of genotoxicity were observed. The tolerance study with 100-times concentrated dose also did not show any statistically significant differences in the assessed parameter...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
(University of Saskatchewan) Scientists from the Vaccine and Infectious Disease Organization-International Vaccine Centre (VIDO-InterVac) at the University of Saskatchewan (USask) and Temple University (Philadelphia, US) have demonstrated that a Salmonella biofilm protein can cause autoimmune responses and arthritis in animals.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Enteric infections caused by Salmonella spp. remain a major public health burden worldwide. Chickens are known to be a major reservoir for this zoonotic pathogen. The presence of Salmonella in poultry farms and a...
Source: Virology Journal - Category: Virology Authors: Tags: Research Source Type: research
st JN Abstract Iron is an essential element for almost all living organisms, which can also be extremely toxic in high concentrations. All organisms must therefore employ homeostatic mechanisms to finely regulate iron uptake, usage and storage in the face of dynamic environmental conditions. The critical step in mammalian systemic iron homeostasis is the fine regulation of dietary iron absorption. However, since the gastrointestinal system is also home to>1014 bacteria, all of which engage in their own programs of iron homeostasis, the gut represents an anatomical location where the inter-kingdom fight for iron...
Source: Immunology - Category: Allergy & Immunology Authors: Tags: Immunology Source Type: research
Salmonellosis is a foodborne disease caused by Salmonella spp. Although cell culture is the gold standard for its identification, validated molecular methods are becoming an alternative, because of their rapidity, selectivity, and specificity. A simplex and duplex droplet digital polymerase chain reaction (ddPCR)-based method for the identification and quantification of Salmonella using ttr, invA, hilA, spaQ, and siiA gene sequences was validated. The method has high specificity, working interval between 8 and 8,000 cp/μL in ddPCR reaction, a limit of detection of 0.5 copies/μL, and precision ranging between 5 and 10...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, the pathogenicity and antibiotic resistance of a representative S. Rissen isolate were investigated. The cell model results showed that S. Rissen preferred to replicate in human macrophage cells U937 compared to murine macrophage cells RAW264.7, suggesting that it has a level of host adaptability. Genome sequencing and comparison analysis revealed that the distribution and nonsynonymous single nucleotide polymorphisms of virulence factors in S. Rissen were similar to those in S. Typhi rather than to those in S. Typhimurium. Taken together, our results suggest that although S. Rissen is a common serotype dist...
Source: G3: Genes Genomes Genetics - Category: Genetics & Stem Cells Authors: Tags: Genome Report Source Type: research
The nucleosome is the primary unit of chromatin structure and commonly imputed as a regulator of nuclear events, although the exact mechanisms remain unclear. Recent studies have shown that certain nucleosomes can have different sensitivities to micrococcal nuclease (MNase) digestion, resulting in the release of populations of nucleosomes dependent on the concentration of MNase. Mapping MNase sensitivity of nucleosomes at transcription start sites genome-wide reveals an important functional nucleosome organization that correlates with gene expression levels and transcription factor binding. In order to understand nucleosom...
Source: G3: Genes Genomes Genetics - Category: Genetics & Stem Cells Authors: Tags: Genome Report Source Type: research
Source: Infection and Drug Resistance - Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
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