MicroRNA ‑28 promotes the proliferation of non‑small‑cell lung cancer cells by targeting PTEN.

MicroRNA‑28 promotes the proliferation of non‑small‑cell lung cancer cells by targeting PTEN. Mol Med Rep. 2020 Mar 20;: Authors: Cui F, Zhou Q, Xiao K, Qian H Abstract Non-small-cell lung cancer (NSCLC) is the fundamental form of lung cancer and the leading cause of cancer‑related mortality in humans. Numerous studies have identified a role for microRNAs (miRs) in cell proliferation, invasion and metastasis in numerous types of cancer, including lung cancer. In the present study, the functional roles and molecular mechanisms of miR‑28 in NSCLC tumorigenesis were investigated. Reverse transcription‑quantitative PCR (RT‑qPCR) was used to measure miR‑28 expression levels in NSCLC tumor tissues and cell lines. A dual‑luciferase assay was performed to observe the direct interaction between miR‑28 and PTEN in A549 cells. Furthermore, the effect of miR‑28 on the mRNA and protein expression levels of PTEN was examined by RT‑qPCR and western blotting, respectively. A Cell Counting kit‑8 assay was performed to identify the relationship between the miR‑28/PTEN axis and tumor cell proliferation using cells infected with lentivirus (LV)‑anti‑miR‑28 or LV‑anti‑miR‑28 + short hairpin RNA‑PTEN. miR‑28 expression was upregulated in NSCLC tumor tissues and cell lines compared with the control groups. PTEN was identified as the downstream gene of miR‑28 in NSCLC and was negatively regulated by miR‑28....
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research