How non-invasive in vivo cell tracking supports the development and translation of cancer immunotherapies.

We describe the principles of imaging host T-cells and adoptively transferred therapeutic T-cells as well as the value of traceable cancer cell models in immunotherapy development. Our emphasis is on in vivo cell tracking methodology, including important aspects and caveats specific to immunotherapies. We discuss a variety of associated experimental design aspects including parameters such as cell type, observation times and intervals, and detection sensitivity. The focus is on preclinical cell tracking but aspects relevant for clinical translation are also discussed.
Source: Frontiers in Physiology - Category: Physiology Source Type: research