Characterization of vaginal microbiota associated with pregnancy outcomes of artificial insemination in dairy cows.

Characterization of vaginal microbiota associated with pregnancy outcomes of artificial insemination in dairy cows. J Microbiol Biotechnol. 2020 Mar 27;: Authors: Chen SY, Deng F, Zhang M, Jia X, Jia SJ Abstract Profitability of dairy and beef industries have been largely affected by the actually achieved reproductive efficiency. Although a large proportion of cows worldwide have been bred by artificial insemination (AI) services, many potential factors affecting the pregnancy outcome of AI have remained to be addressed. In the present study, we investigated the vaginal microbiota by high-throughput sequencing of 16S rRNA gene and analyzed their association with differential pregnancy outcomes (i.e., pregnant vs. nonpregnant) of multiple AI services in dairy cows. Sequencing of V3-V4 region totally produced 512,046 high-quality sequences that were computationally clustered into 2,584 operational taxonomic units (OTUs). All OTUs were taxonomically assigned to 10 bacterial phyla. There were statistically significant differences among the three times of AI service (T1, T2 and T3) with respective to Shannon index and number of observed OTUs (P < 0.05). Bray-Curtis distance-based PCoA analysis also revealed that T2 group could be significantly distinguished from T1 and T3. However, no significant difference between the pregnant and nonpregnant cows was found in confidence regarding both alpha diversity and beta diversity. These results...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research