Double crosslinked HLC-CCS hydrogel tissue engineering scaffold for skin wound healing.

Double crosslinked HLC-CCS hydrogel tissue engineering scaffold for skin wound healing. Int J Biol Macromol. 2020 Mar 30;: Authors: Cao J, Wang P, Liu Y, Zhu C, Fan D Abstract Skin defects caused by various reasons are currently common clinical problems. At present, hydrogels have been proposed as tissue-engineered skin scaffolds to regenerate the tissues of the defect. We used human-like collagen (HLC), which was isolated and purified after high-density fermentation of recombinant E. coli BL21 The gel uses HLC and carboxymethylated chitosan (CCS) as raw materials and combines enzyme-chemical double cross-linking technology to form a three-dimensional porous network structure that mimics the human extracellular matrix, providing attachment points and nutrients for cell growth and proliferation. For comparison, we used a common hydrogel raw material, gelatin, to prepare a hydrogel in the same way. The experimental results show that the HLC-CCS skin scaffold hydrogel has good mechanical properties, high porosity and good histocompatibility. And full-thickness skin defect repair experiments show that this hydrogel has a good ability to promote skin tissue regeneration at the wound. In summary, this HLC-based double-crosslinked hydrogel can be used as a project strategy for skin defect repair. PMID: 32240736 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research

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Publication date: Available online 22 May 2020Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Jorge Serment-Guerrero, Viridiana Dominguez-Monroy, Jenny Davila-Becerril, Enrique Morales-Avila, Jorge Fuentes-Lorenzo
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research
AlkB is a bacterial Fe(II)– and 2-oxoglutarate–dependent dioxygenase that repairs a wide range of alkylated nucleobases in DNA and RNA as part of the adaptive response to exogenous nucleic acid–alkylating agents. Although there has been longstanding interest in the structure and specificity of Escherichia coli AlkB and its homologs, difficulties in assaying their repair activities have limited our understanding of their substrate specificities and kinetic mechanisms. Here, we used quantitative kinetic approaches to determine the transient kinetics of recognition and repair of alkylated DNA by AlkB. These ...
Source: Journal of Biological Chemistry - Category: Chemistry Authors: Tags: DNA and Chromosomes Source Type: research
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Source: JPEN Journal Of Parenteral And Enteral Nutrition - Category: Nutrition Authors: Tags: JPEN J Parenter Enteral Nutr Source Type: research
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Source: Chembiochem - Category: Biochemistry Authors: Tags: Chembiochem Source Type: research
Authors: Kuznetsov NA, Fedorova OS Abstract X-ray data show that DNA glycosylases, which initiate the pathway of base excision repair in DNA, belong to six structural superfamilies. Here, we provide an overview of the latest results of kinetic studies on the mechanisms of specific recognition of a damaged nucleotide at the early steps of DNA repair by human (OGG1 and MBD4) or Escherichia coli (Nth and MutY) N-DNA-glycosylases belonging to superfamily Helix-hairpin-Helix (HhH). A comparison of real-time conformational transformations of DNA glycosylases and DNA with the structural data obtained for free enzymes and ...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
Abstract Effects of environmental factors for growth of Escherichia coli on spontaneous mutagenesis and homologous recombination events are described. By analyzing rifampicin-resistant (Rifr) mutation frequencies in an E. coli strain lacking MutM and MutY repair enzymes, which suppress base substitution mutations caused by 8-oxoguanine (7,8 dihydro-8-oxoguanine; 8-oxoG) in DNA, we examined levels of oxidative DNA damage produced in normally growing cells. The level of 8-oxoG DNA damage was about 9- and 63-fold higher in cells grown in M9-glucose and M9-glycerol media, respectively, than in those grown in LB medium...
Source: Genes and Genetic Systems - Category: Genetics & Stem Cells Authors: Tags: Genes Genet Syst Source Type: research
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Source: eLife - Category: Biomedical Science Tags: Developmental Biology Neuroscience Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract Developing wound dressing that inhibits bacterial infection for treating complex wound healing processes has been a research hotspot. Here, we report the fabrication of Cu-MOFs (HKUST-1) incorporated electrospun chitosan/polyvinyl alcohol (HKUST-1/chitosan/PVA) fibers through the blending electrospinning for wound therapy. HKUST-1/chitosan/PVA fibers displayed satisfying physical properties, such as mechanical property, water uptake, water vapor transmission rate, etc. Cytotoxicity test indicated that HKUST-1/chitosan/PVA fibers were biocompatible and could support cell adhesion. Due to the HKUST-1 incorp...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
co In Escherichia coli, DNA replication forks stall on average once per cell cycle. When this occurs, replisome components disengage from the DNA, exposing an intact, or nearly intact fork. Consequently, the fork structure must be regressed away from the initial impediment so that repair can occur. Regression is catalyzed by the powerful, monomeric DNA helicase, RecG. During this reaction, the enzyme couples unwinding of fork arms to rewinding of duplex DNA resulting in the formation of a Holliday junction. RecG works against large opposing forces enabling it to clear the fork of bound proteins. Following subsequent pr...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Review Source Type: research
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