Regioselective sulfation of β-glucan from Ganoderma lucidum and structure-anticoagulant activity relationship of sulfated derivatives.

Regioselective sulfation of β-glucan from Ganoderma lucidum and structure-anticoagulant activity relationship of sulfated derivatives. Int J Biol Macromol. 2020 Mar 30;: Authors: Zhang Z, Tang Q, Wu D, Zhou S, Yang Y, Feng N, Tang C, Wang J, Yan M, Liu Y, Zhang J Abstract In the present study, regioselective sulfation of β-glucan (GLP) from Ganoderma lucidum were firstly established by using 4,4'-dimethoxytrityl chloride and hexamethyldisilazane as protecting precursor. 2,4,6-O-sulfated, 6-O-sulfated and 2,4-O-sulfated GLP derivatives were prepared and the molecular weights (Mw) of derivatives were determined to range from 0.94 × 104 to 6.27 × 104 g/mol, while the degrees of sulfation (DS) were calculated to vary from 0.83 to 1.74. The regioselective sulfation of GLP was confirmed by FT-IR, 13C NMR spectroscopy and methylation analysis. Results indicated that the sulfated substitution sites were predominantly at C-6 in 6-O-sulfated GLP (S6-OGLP) and C-4 in 2,4-O-sulfated GLP (S2,4-OGLP), respectively. Clotting assays (APTT, PT and TT) in vitro showed that sulfate groups were essential for anticoagulant activity and S6-OGLP exhibited much higher than others. Meanwhile, sulfated GLP with higher DS and Mw showed stronger anticoagulant activity in the case of the same condition. PMID: 32240743 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research