Molecules, Vol. 25, Pages 1643: Acetylcholinesterase Inhibitors among Zingiber officinale Terpenes —Extraction Conditions and Thin Layer Chromatography-Based Bioautography Studies
Molecules, Vol. 25, Pages 1643: Acetylcholinesterase Inhibitors among Zingiber officinale Terpenes—Extraction Conditions and Thin Layer Chromatography-Based Bioautography Studies
Molecules doi: 10.3390/molecules25071643
Authors:
Lidia Czernicka
Agnieszka Ludwiczuk
Edward Rój
Zbigniew Marzec
Agata Jarzab
Wirginia Kukula-Koch
Although numerous studies have been conducted on ginger extracts and fractions, the data on the pharmacological activity of single constituents of Zingiber officinale are still insufficient. To assess the antidementia properties of the plant, a thin layer chromatography (TLC)-based bioautography acetylcholinesterase inhibitory assay was performed on the Zingiber officinale diethyl ether extract. It led to the recognition of three active inhibitors among volatile constituents of the plant: ar-curcumene (A), α-sesquiphellandrene (B) and a-zingiberene (C). The identification of the components was possible thanks to the application of a TLC–HPLC-MS interface analysis of active zones and the GC-MS qualitative analysis of the tested samples. Based on the obtained results, the influence of several extraction techniques (hydrodistillation—HD, pressurized liquid extraction or accelerated solvent extraction—ASE, shaking maceration–SM, supercritical fluid extraction–SFE, and ultrasound-assisted extraction—UAE) on the recovery of the active metabolite...
Source: Molecules - Category: Chemistry Authors: Lidia Czernicka Agnieszka Ludwiczuk Edward R ój Zbigniew Marzec Agata Jarzab Wirginia Kukula-Koch Tags: Article Source Type: research