Interleukin-1 Ligands and Receptors in Lumpfish (Cyclopterus lumpus L.): Molecular Characterization, Phylogeny, Gene Expression, and Transcriptome Analyses

The interleukin (IL)-1 family play a fundamental role as immune system modulators. Our previous transcriptome-analyses of leukocytes from lumpfish (Cyclopterus lumpus L.) showed that IL-1β was among the most highly upregulated genes following bacterial exposure. In the present study, we characterized IL-1 signaling pathways, identified and characterized four ligands of the IL-1 family in lumpfish; IL-1β type I and type II, IL-18, and the novel IL-1 family members (nIL-1F), both at mRNA and gene levels. The two IL-1β in lumpfish is termed IL-1β1 (type II) and IL-1β2 (type I). Furthermore, a comprehensive phylogenetic analysis of 277 IL-1 ligands showed that nIL-1F, in common with IL-1β, likely represents an ancestral gene, as representatives for nIL-1F were found in cartilaginous and lobe-finned fish, in addition to teleosts. This shows that nIL-1F is not exclusively present in teleosts as previously suggested. Our analyses of exon-intron structures, intron phases, phylogeny and synteny clearly show the separation of IL-1β into groups; type I and type II, which likely is a result of the third whole genome duplication (3R WGD). The phylogenetic analysis shows that most teleosts have both type I and type II. Furthermore, we have determined transcription levels of the IL-1 ligands in leukocytes and 16 different tissues, and their responses upon in vitro stimulation with seven different ligands. In addition, we have identified the IL-1 receptors ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

Related Links:

Publication date: Available online 11 June 2019Source: Fish &Shellfish ImmunologyAuthor(s): Yeny Leal, Janet Velazquez, Liz Hernandez, Jaya Kumari Swain, Alianet Rodríguez Rodríguez, Rebeca Martínez, Claudia García, Yassel Ramos, Mario Pablo Estrada, Yamila CarpioAbstractThe development of vaccines employing conserved protein antigens, for instance ribosomal protein P0, has as disadvantage the high degree of identity between pathogen and host proteins due to possible induction of tolerance or auto antibodies in the host organism. To overcome this drawback, peptide-based vaccines have been de...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Conclusion Early infectious factors investigated in this study were not associated with MS risk.
Source: Multiple Sclerosis and Related Disorders - Category: Neurology Source Type: research
Rickettsial infections are caused by bacteria from the Rickettsia genus which are often spread by biting ticks, fleas, mites or lice. Rickettsia can cause mild to severe illness and Rickettsia species can be found worldwide. Early stage, nonspecific symptoms of infections can make clinical diagnosis difficult. Rickettsial infection symptoms, if present, typically develop within 1 -2 weeks of exposure and include fever, headache, malaise, rash, nausea, and vomiting. Some rickettsial diseases such as Rocky Mountain spotted fever (RMSF), Mediterranean spotted fever, and typhus fever, can be life threatening and even fatal in ...
Source: NIH OTT Licensing Opportunities - Category: Research Authors: Source Type: research
In this study, we analyse L. salmonis from a time series by RNA sequencing applying a method of separating individuals of different instar age independent of sampling time point. Lice of four stages divided into up to four age groups within the stage were analysed in triplicate (total of 66 samples). Gene expression analysis shows that the method for sorting individuals was successful. Many genes show cyclic expression patterns over the moulting cycles. Overall gene expression differs more between lice of different age within the same stage than between lice of different stage but same instar age.
Source: Journal of Fish Diseases - Category: Zoology Authors: Tags: SPECIAL ISSUE Source Type: research
Publication date: Available online 27 December 2017 Source:Veterinary Parasitology Author(s): Domenico Otranto Vector-borne pathogens have developed a close relationship with blood feeding arthropod ectoparasites (e.g., mosquitoes, ticks, phlebotomine sand flies, black flies, fleas, kissing bugs, lice) and exploited a huge variety of vector transmission routes. Therefore, the life cycles of these pathogens result in a long evolved balance with the respective arthropod biology, ecology and blood feeding habits, instrumentally to the infection of several animal species, including humans. Amongst the many parasite transmissi...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
Abstract Atlantic lumpfish (Cyclopterus lumpus L.) is used as a biological delousing agent for sea lice (Lepeophtheirus salmonis K.) infestations in Norwegian aquaculture. Here, we present a study on the antibody response and vaccine side effects after intramuscular and intraperitoneal injection of lumpfish with two vaccines. Both vaccines contained bacterial antigens from atypical Aeromonas salmonicida A‐layer types V and VI, Vibrio anguillarum serotype O1 and Moritella viscosa sp., but one vaccine contained a vegetable oil‐based adjuvant, while the other contained a mineral oil‐based adjuvant. Intramuscular injecti...
Source: Journal of Fish Diseases - Category: Zoology Authors: Tags: ORIGINAL ARTICLE Source Type: research
The objective of this study was to obtain further information about the health status and health needs of IDP women and children, aged 15 and under, living in the informal settlements in central Iraq. The study was conducted between February and June 2015. Our focus was on settlements in Baghdad, Karbala and Kirkuk, which between them hosted an estimated 688,944 IDPs, about a third of Iraq’s IDPs.14 This estimate is undoubtedly low as some IDPs were not counted or registered. It is the hope that information from this study will help improve access to services for this vulnerable population. These governorates were ...
Source: PLOS Currents Disasters - Category: Global & Universal Authors: Source Type: research
We're still trying to eradicate diseases that have plagued humans for centuries. References to malaria, for instance, have been found in recorded history in 2700 BC China. Riaan Rifkin, a prehistoric archaeologist, together with geneticists at the University of Pretoria, South Africa gets down to the root of origins of human disease pathogens in sub-Saharan Africa, the cradle of mankind. He and his team are trying to pin down the origins of human disease and establish a sub-Saharan African disease baseline that precedes the exodus of Homo sapiens from Africa 65000 years ago and, perhaps, to also assist in the discovery of ...
Source: Science - The Huffington Post - Category: Science Source Type: news
Publication date: Available online 27 February 2016 Source:Preventive Veterinary Medicine Author(s): Alexander G. Murray, Maya Wardeh, K. Marie McIntyre Atlantic salmon’s (Salmo salar) annual aquaculture production exceeds 2M tonnes globally, and for the UK forms the largest single food export. However, aquaculture production is negatively affected by a range of different diseases and parasites. Effort to control pathogens should be focused on those which are most “important” to aquaculture. It is difficult to specify what makes a pathogen important; this is particularly true in the aquatic sector wh...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
ConclusionsDisease prevention measures such as vaccination, quarantine and participation in systematic control schemes were used by a minority of respondents. The results suggest that respondents acknowledge BVD as an important and relevant disease, despite many believing it was not a problem in their herd. Interest in BVD appears to be high and it is likely that an education program would be well received.
Source: Australian Veterinary Journal - Category: Veterinary Research Authors: Tags: PRODUCTION ANIMALS Source Type: research
More News: Allergy & Immunology | Fish | Genetics | Lice | Study | Vaccines