Effect of Modified Xijiao Dihuang Decoction ( 加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis

AbstractObjectiveTo observe the effect of Modified Xijiao Dihuang Decoction ( 加味犀角地黄汤, MXDD) on rats with radiation enteritis, and explore its action mechanism.MethodsThirty female Sprague Dawley rats were divided into the control, model, dexamethasone (DXM), golden bifid (GB) and MXDD groups using random number table, 6 rats in each group. Except the control group, the other rats were developed into radiation enteritis model by exposing to a single60Co- γ ray at a dose of 11 Gy. The rats in the DXM, GB and MXDD groups were treated with DXM (1.425 mg/kg), GB (0.8 g/kg) and MXDD (36.0 g/kg) for 3 days, respectively. Body weight and diarrhea condition of rats were evaluated daily. On day 3, the feces of rats were collected for intestinal flora detect ion and the small intestinal tissues were also collected. Bacterial species annotation, alpha and beta diversities as well as composition of intestinal flora were detected and compared. The protein and mRNA expressions of interleukin 17 (IL-17), retinoid-related orphan nuclear receptor gamma t (ROR- γt) and forkhead/ winged helix transcription factor p3 (FoxP3) were determined by Western blot and polymerase chain reaction, respectively. The abundance and diversity of intestinal flora as well as the proportion at the phylum and genus levels were assayed by 16S rRNA metagenome sequencing. Correl ation between intestinal flora and Th17/Treg was analyzed by heatmap method.ResultsOn day 1 to 3 after radiation, ...
Source: Chinese Journal of Integrative Medicine - Category: Internal Medicine Source Type: research

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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Archives of Microbiology - Category: Microbiology Authors: Tags: Arch Microbiol Source Type: research
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