Cancers, Vol. 12, Pages 860: The Calcium-Sensing Receptor is A Marker and Potential Driver of Neuroendocrine Differentiation in Prostate Cancer
Cancers, Vol. 12, Pages 860: The Calcium-Sensing Receptor is A Marker and Potential Driver of Neuroendocrine Differentiation in Prostate Cancer
Cancers doi: 10.3390/cancers12040860
Authors:
Fanny Bery
Mathilde Cancel
Aurélie Chantôme
Roseline Guibon
Franck Bruyère
François Rozet
Karine Mahéo
Gaëlle Fromont
The mechanisms underlying neuroendocrine (NE) differentiation in prostate cancer (PCa) remain mostly uncharacterized. Since a deregulated calcium homeostasis has been reported in neuroendocrine prostate cancer (NEPC), we explored herein the link between NE differentiation and the calcium-sensing receptor (CaSR). CaSR expression was evaluated by immunohistochemistry—together with NE markers—on tissue microarrays containing samples of normal prostate, localized PCa, metastatic castration resistant PCa (MCRPC) and NEPC. In prostate tissues, we observed a strong association between CaSR and chromogranin expression. Both markers were strongly expressed in all cases of NEPC and co-expression was confirmed by double immunostaining. In MCRPC, the expression of CaSR was significantly associated with shorter overall survival. The involvement of CaSR in NE differentiation was evaluated in PCa cell lines. Inhibition of CaSR led to decrease the expression of neuronal (NSE, βtubulinIII) and NE (chromogranin, synaptophysin) markers in the NE PCa cell line NCI-H660. A decrease of neuronal and NE markers was also observed ...
Source: Cancers - Category: Cancer & Oncology Authors: Fanny Bery Mathilde Cancel Aur élie Chantôme Roseline Guibon Franck Bruy ère Fran çois Rozet Karine Mah éo Ga ëlle Fromont Tags: Article Source Type: research