GSE140351 Capability of FDA-EVIR Microarray for Detection of Norovirus and Hepatitis A Virus in Inoculated Tomatoes, Green Onions, and Celery
Contributors : Christine Yu ; Kaoru Hida ; Efstathia PapafragkouSeries Type : Expression profiling by genome tiling arrayOrganism : Allium fistulosum ; Apium graveolens ; Astrovirus sp. ; Bacillus subtilis ; Coxsackievirus ; Hepatitis E virus ; Hepatovirus A ; Norovirus ; Rotavirus ; Sapovirus ; Solanum lycopersicum ; unidentified adenovirusThe capability of the U.S. Food and Drug Administration Enteric Viruses tiling microarray (FDA-EVIR) was assessed for rapid molecular identification of human norovirus (NoV) and hepatitis A virus (HAV) extracted from artificially inoculated fresh produce. Two published viral extraction strategies, total RNA extraction or virus particle isolation, were employed to prepare the viral targets. We also assessed the amount of viral RNA extracted from celery by three commercially-available kits and how well that RNA performed on the FDA-EVIR. Our results confirm that FDA-EVIR can correctly identify common enteric viruses isolated from fresh produce and is capable of identifying single and mixed species of viruses, as well as distinguishing among genotypes. Extending microarray methods to other food matrices should provide important support to surveillance and outbreak investigations.
Publication date: Available online 30 May 2020Source: Nutrition, Metabolism and Cardiovascular DiseasesAuthor(s): Anna Vittoria Mattioli, Susanna Sciomer, Camilla Cocchi, Silvia Maffei, Sabina Gallina
Government leaders in the U.S. are warning that the massive protests following the death of George Floyd by police in Minneapolis could fuel a new surge in coronavirus cases
The store had reopened Friday after being closed due to the coronavirus outbreak. But it closed its doors Saturday because of George Floyd protests.
Publication date: Available online 30 May 2020Source: American Journal of Infection ControlAuthor(s): Byung-Han Ryu, Younghwa Cho, Oh-Hyun Cho, Sun In Hong, Sunjoo Kim, Seungjun Lee
Cities across the U.S. smolder from protests over police violence, in which virus precautions were uneven. Nicaragua has become a place of midnight burials.
Publication date: August 2020Source: Pharmacological Research, Volume 158Author(s): Brigida Boccanegra, Ingrid E.C. Verhaart, Ornella Cappellari, Elizabeth Vroom, Annamaria De Luca
Two hospitals for coronavirus patients open in Istanbul as Turkey’s daily number of new cases falls to its lowest since the peak of the outbreak
Publication date: July 2020Source: Molecular Immunology, Volume 123Author(s): Ran Mo, Xin-Xia Feng, Ya-Nan Wu, Han Wang, Yong-Pei He, Huan-Huan Sun, Fang Guo, Qian Chen, Wei Yan, Pei-Yuan Li, Mei Liu, Gui-Mei Zhang, De-An Tian, Zuo-Hua Feng
Abstract One-year surveillance for enteric viruses in raw sewage was conducted in Kansai area, central part of Japan from July 2015 to June 2016. The raw sewage was collected monthly from an inlet polluted pool and was concentrated by polyethylene glycol (PEG) precipitation. Twelve sewage samples were screened for nineteen kinds of enteric viruses by using RT-PCR method and further analyzed by nucleotide sequencing. Twelve enteric viruses were found in the investigative sewage samples. Rotavirus A and norovirus GI and GII with several genotypes were detected all year round. Interestingly, norovirus GII.17 (Kawasak...
By: Rachael Rettner, Senior Writer Published: 08/03/2016 06:02 PM EDT on LiveScience The coastal waters around Rio de Janeiro, where many Olympic water competitions will soon take place, are reportedly teeming with harmful viruses and bacteria. So what illnesses might people catch if they swallow some of the water? If the water has been contaminated with raw sewage, as has been reported, then a number of common pathogens could be lurking there and make people ill, experts say. “There are many types of microbes in raw sewage that have the potential to cause human disease,” said Stephen Morse, a professor of epid...