Source: Cancer Medicine - Category: Cancer & Oncology Authors: Qi Wang,
Sean X. Leng,
Haiyan ZhangTags: ORIGINAL RESEARCH Source Type: research
According to a study published inThe Journal of Clinical Endocrinology& Metabolism, obesity is not associated with an increased risk of developing thyroid disease or thyroid cancer.Endocrinology Advisor
Source: Anti-Cancer Agents in Medicinal Chemistry - Category: Cancer & Oncology Authors: Islam, Muhammad T. Ali, Eunus S. Uddin, Shaikh J. Khan, Ishaq N. Shill, Manik C. de Castro e Sousa, Jo ão M. de Alencar, Marcus V.O. Barros Melo-Cavalcante, Ana A.C. Mubarak, Mohammad S.Tags: Review article Source Type: research
Source: Anti-Cancer Agents in Medicinal Chemistry - Category: Cancer & Oncology Authors: Li, Jun Li, Jie Zhang, Jiaojiao Shi, Jiantao Ding, Shi Liu, Yajing Chen, Ye Liu, JuTags: Research article Source Type: research
In this study we adopted an all-in-one method. Primers designed according to the two gRNA sequences and the tracRNA-U6 vector sequences were used to produce a gRNA1-tracRNA-U6-gRNA2 fragment. Then the proper fragment was ligated into the lentiCRISPRv2 vector and verified by sequencing.
Lentiviral Packaging and Stable Cell Line Screening
The constructed eukaryotic expression vector and gene knockdown vector were transfected into the 293T cells with the packaging plasmids psPAX2 and pMD2.G using Lipofectamine 2000 (Thermo Fisher, Massachusetts, USA). The lentivirus was harvested 48 h after transfection, and the lentivirus ...