Long Non-coding RNA THRIL Mediates Cell Growth and Inflammatory Response of Fibroblast-Like Synoviocytes by Activating PI3K/AKT Signals in Rheumatoid Arthritis

AbstractThe present study explored the possible functions and the underlying mechanism of tumor necrosis factor- α (TNF-α) and heterogeneous nuclear ribonucleoprotein L (hnRNPL)–related immunoregulatory lncRNA plasmacytoma variant translocation 1 (THRIL) in rheumatoid arthritis (RA). Serum samples were collected from patients with RA. Primary fibroblast-like synoviocytes (FLSs) were separated from synovial tissues and cultured for subsequent cell experiments by transfecting different vectors. The qRT-PCR analysis was employed for evaluating the levels of THRIL in the serum. Enzyme-linked immunosorbent assay (ELISA) analysis was employed to detect the levels of inflammatory cytokines. MTT assay and An nexin V-FITC/PI apoptosis assay were used to evaluate the cell viability and apoptosis, respectively. Besides, the levels of the apoptotic proteins and the pathway-related proteins were measured by western blotting. Pearson’s correlation analysis was used to assess the correlation between THRIL an d clinical parameters. THRIL was overexpressed in the blood of patients with RA as compared with healthy participants (p 
Source: Inflammation - Category: Allergy & Immunology Source Type: research

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The monoclonal interleukin-17A antagonist has three other indications: ankylosing spondylitis in adults, psoriatic arthritis in adults, and plaque psoriasis in adults and children age 6 and older.Medscape Medical News
Source: Medscape Dermatology Headlines - Category: Dermatology Tags: Rheumatology News Source Type: news
Condition:   Chronic Lower Back Pain Intervention:   Device: PICO G2 4k Sponsors:   Cedars-Sinai Medical Center;   National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Recent research found women with COPD and asthma—even those who have never smoked—are at a higher risk of developing rheumatoid arthritis...
Source: The Rheumatologist - Category: Rheumatology Authors: Tags: Conditions Rheumatoid Arthritis 2019 ACR/ARP Annual Meeting asthma chronic obstructive pulmonary disorder COPD lungs Source Type: research
Contributors : Sel çuk ÖZDEMÄ°R ; Serdar ALTUNSeries Type : Expression profiling by high throughput sequencing ; Non-coding RNA profiling by high throughput sequencingOrganism : Bos taurusMycoplasma bovis (M. bovis) causes to the diseases such as arthritis, pneumonia, abortion and mastitis causing to great losses in dairy bovine industries. It undertakes significant functions in the regulation of the immune response formed by many RNA type bacteria such as Messenger RNA (mRNAs) and Long noncoding RNA (lncRNAs). mRNA and lncRNA expression profiles still cannot be majorly known in the bovine mammary gland tissues...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing Bos taurus Source Type: research
(Uppsala University) Researchers at Uppsala University and Uppsala University Hospital have developed a new method to measure levels of the medication hydroxychloroquine in patients with the rheumatic disease systemic lupus erythematosus (SLE). The analysis method may also be useful in other areas, such as in the treatment of COVID-19. The study is being published in Arthritis Research and Therapy.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Publication date: Available online 30 May 2020Source: Journal of Hazardous MaterialsAuthor(s): Jun Ge, Haixin Wang, Jiayu Lin, Shengke Tian, Jianqi Zhao, Xianyong Lin, Lingli Lu
Source: Journal of Hazardous Materials - Category: Environmental Health Source Type: research
Objective: To assess the efficacy and safety profiles of different dosing regimens of tofacitinib, baricitinib, and upadacitinib, novel selective oral Janus activated kinase inhibitors, in rheumatoid arthritis (RA).
Source: Mayo Clinic Proceedings - Category: Internal Medicine Authors: Tags: Original article Source Type: research
Abstract Deltex-3-like (DTX3L), an E3 ligase, which is also known as B-lymphoma and BAL-associated protein (BBAP), is a member of the Deltex (DTX) family and was originally identified as a binding partner of diphtheria-toxin-like ADP-ribosyltransferase-9 (ARTD9). The present study found that DTX3L and ARTD9 were upregulated in synovial tissues obtained from rheumatoid arthritis (RA) patients compared with those from the controls. Healthy synovial tissues were obtained by arthroscopic biopsy from patients with meniscus injury (n = 10 samples) without a history of RA in the Orthopedic Department of the...
Source: Tissue and Cell - Category: Cytology Authors: Tags: Tissue Cell Source Type: research
FUS is a nucleic acid binding protein that, when mutated, cause a subset of familial amyotrophic lateral sclerosis (ALS). Expression of FUS in yeast recapitulates several pathological features of the disease-causing mutant proteins, including nuclear to cytoplasmic translocation, formation of cytoplasmic inclusions, and cytotoxicity. Genetic screens using the yeast model of FUS have identified yeast genes and their corresponding human homologs suppressing FUS induced toxicity in yeast, neurons and animal models. To expand the search for human suppressor genes of FUS induced toxicity, we carried out a genome-scale genetic s...
Source: G3: Genes Genomes Genetics - Category: Genetics & Stem Cells Authors: Tags: Mutant Screen Report Source Type: research
Publication date: Available online 30 May 2020Source: The Journal of Foot and Ankle SurgeryAuthor(s): Derek S. Anselmo, Lisa Thatcher, David Erfle
Source: The Journal of Foot and Ankle Surgery - Category: Orthopaedics Source Type: research
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