Prevalence of fosfomycin resistance genes and antimicrobial susceptibility of clinical urinary extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates

The antimicrobial susceptibility testing of 284 Enterobacteriaceae isolates responsible for urinary tract infections to ampicillin, ceftazidime, ciprofloxacin, nitrofurantoin, trimethoprin-sulfamethaxole, and fosfomycin was performed by disk diffusion method. Additionally, in fosfomycin-resistant and intermediate susceptible isolates using disk diffusion method, the minimum inhibitory concentration (MIC) of fosfomycin was determined by agar dilution. The presence of fosA and fosA3 genes and ESBL genes was investigated in fosfomycin-resistant isolates and ESBL-producing isolates, respectively. Klebsiella pneumoniae [72.34% (34/47)], and Escherichia coli [58.23% (138/237)] were identified as ESBL-producers by phenotype. These isolates were positive for the presence of blaTEM, blaSHV, blaCTX-M genes in 56.97% (n = 98), 55.23% (n = 95), and 78.48% (n = 135), respectively. Resistance to fosfomycin was detected in 9.4% (13/138) and 5.9% (2/34) of the ESBL-E. coli and ESBL-K. pneumoniae isolates. In fosfomycin-resistant isolates, the presence of the fosA gene was detected in two ESBL-E. coli isolates; the fosA3 gene in one non-ESBL E. coli isolate; and the fosA3 gene co-harboured with blaCTX-M in six ESBL-E. coli (n = 5) and one K. pneumoniae isolates. A high prevalence of ESBL-producing Enterobacteriaceae (72.6%) was detected in urine samples. Fosfomycin showed the highest susceptibility rate (≥87%) of antimicrobials tested in ESBL-producing E. coli and K. pneumo...
Source: Reviews in Medical Microbiology - Category: Microbiology Tags: BACTERIOLOGY Source Type: research

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CONCLUSION: The study highlights the prevalence of PMQR genes in E. coli with high association with ESBL phenotype. This finding is a sign of limited therapeutic options for E. coli. PMID: 32433009 [PubMed - as supplied by publisher]
Source: Infectious Disorders Drug Targets - Category: Infectious Diseases Authors: Tags: Infect Disord Drug Targets Source Type: research
Publication date: June 2020Source: Journal of Global Antimicrobial Resistance, Volume 21Author(s): Fatemeh Mottaghizadeh, Hanieh Mohajjel Shoja, Mehri Haeili, Davood Darban-Sarokhalil
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Conclusions: Among the most common pathogens isolated from hospitalized patients with cUTI, the rates of single resistance to common treatments and of cross-resistance to these regimens are substantial. Knowing the patterns of cross-resistance may help clinicians tailor empiric therapy more precisely. PMID: 32423953 [PubMed - as supplied by publisher]
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
In conclusion, the most interesting finding is the association of iss with septic shock. Adhesins are important for UTI pathogenesis, while otherwise low-pathogenic isolates from the microbiota can cause post-prostate biopsy sepsis. PMID: 32423949 [PubMed - as supplied by publisher]
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Microbial Drug Resistance, Ahead of Print.
Source: Microbial Drug Resistance - Category: Microbiology Authors: Source Type: research
In this study, we aimed to investigate the burden of antimicrobial resistance and occurrence of ESBL genes among clinical isolates of uropathogenic Escherichia coli at a tertiary care teaching hospital of Nepal. During the study period, we processed a total of 1,626 urinary tract specimens, isolated significant bacterial pathogens, and investigated their antimicrobial susceptibilities. Escherichia coli (n = 154), the predominant pathogen associated with UTI, was further investigated for the existence of ESBL enzymes by using conventional phenotypic as well as molecular approaches. Among suspected cases of UTI...
Source: Interdisciplinary Perspectives on Infectious Diseases - Category: Infectious Diseases Tags: Interdiscip Perspect Infect Dis Source Type: research
Authors: Tigabu A, Ferede W, Belay G, Gelaw B Abstract Background: Urinary tract infections are the common types of infections in the community and health care settings. Despite the widespread availability of antibiotics, urinary tract infection remains a worldwide therapeutic problem. It is a continuous and significant problem in cancer patients. Methods: A hospital-based comparative cross-sectional study was conducted on 240 study participants from January to June 2019. Sociodemographic data were collected by a predesigned questionnaire and midstream urine samples collected using simple random sampling techni...
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
Authors: Rosana Y, Ocviyanti D, Halim M, Harlinda FY, Amran R, Akbar W, Billy M, Akhmad SRP Abstract Pregnant women are usually at risk of urinary tract infections (UTIs) such as asymptomatic bacteriuria. In the current multidrug-resistance era, appropriate diagnosis and treatment should be provided to avoid complications in pregnant women in developing countries, which have limited facilities, such as Indonesia. The aim of this study was to evaluate in vitro susceptibility tests. Urinary isolates were collected from 715 pregnant women who visited eight Community Health Centers in Jakarta, Indonesia, between 2015 a...
Source: Infectious Diseases in Obstetrics and Gynecology - Category: OBGYN Tags: Infect Dis Obstet Gynecol Source Type: research
Microbial Drug Resistance, Ahead of Print.
Source: Microbial Drug Resistance - Category: Microbiology Authors: Source Type: research
Publication date: Available online 4 May 2020Source: IDCasesAuthor(s): Paige M.K. Larkin, Susan E. Realegeno, Kevin W. Ward, Omai B. Garner, Shangxin Yang
Source: IDCases - Category: Infectious Diseases Source Type: research
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