GSE132758 Single Cell Gene Expression Analysis Reveals Human Stem Cell-Derived Graft Composition in a Cell Therapy Model of Parkinson ’s Disease

Contributor : Yogita SharmaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensSince the pioneering studies using fetal cell transplants in Parkinson ’s disease (PD), brain repair by cell replacement has remained a long-standing and realistic goal for the treatment of neurodegenerative disorders including PD. Authentic and functional dopamine (DA) neurons can now be generated from human pluripotent stem cells (hPSCs), and these cell preparation s are both safe and functional when transplanted to animal models of PD1. However, although resulting grafts from fetal brain tissue and hPSCs contain large numbers of desired DA neurons, these therapeutic cells are a minor component of the grafts. Moreover, the cellular composition of the graft has remained difficult to assess due to limitations in histological methods that rely on pre-conceived notions concerning cell types. Here, we used single cell RNA sequencing (scRNA-seq) to characterize intracerebral grafts of fetal and ventral midbrain (VM)-patterned human embryonic stem cells (hESCs) after long-term survival and functional maturation in a pre-clinical rat model of PD.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

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