SMAD3 promotes ELK3 expression following transforming growth factor β-mediated stimulation of MDA-MB231 cells.

SMAD3 promotes ELK3 expression following transforming growth factor β-mediated stimulation of MDA-MB231 cells. Oncol Lett. 2020 Apr;19(4):2749-2754 Authors: Park JH, Park KS Abstract Transforming growth factor-β (TGFβ) is a secreted cytokine whose aberrant spatiotemporal expression is related to cancer progression and metastasis. While TGFβ acts as a tumor suppressor in normal and premalignant stages, TGFβ functions as a tumor promoter during the malignant phases of tumor progression by prompting cancer cells to undergo epithelial-mesenchymal transition (EMT), which enhances tumor cell invasion and ultimately promotes metastasis to other organs. Extensive studies have been performed to uncover the molecular and cellular mechanisms underlying TGFβ inducing EMT in cancer cells. Here, we suggested that ELK3, which encodes a protein that orchestrates invasion and metastasis of triple negative breast cancer cells, is a downstream target of TGFβ-SMAD3 in MDA-MB231 cells. ELK3 expression was increased in a time-dependent manner upon TGFβ treatment. Chemical and molecular inhibition of the TGFβ receptor blocked the ability of TGFβ to induce ELK3 expression. Small interfering RNA-mediated suppression analysis revealed that SMAD3 induces TGFβ signaling to express ELK3. Moreover, the results of the luciferase reporter assay and chromatin immunoprecipitation analysis showed that SMAD3 directly binds to the SMAD-b...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

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When you’re tearing off a sheet of aluminum foil to cook with — or wrap your leftovers before you put them in the fridge — you probably never consider aluminum is a dangerous toxin. Truth is, aluminum is a heavy metal… just like lead, mercury and arsenic. It’s in our food… our cookware… even in the water we drink. Exposure to aluminum is a risk factor for cancer, Alzheimer’s, Parkinson’s, and bone disease, just to name a few. Our lungs, liver, and kidneys are able to eliminate a majority of the small amounts of aluminum we ingest through eating, drinking or breath...
Source: Al Sears, MD Natural Remedies - Category: Complementary Medicine Authors: Tags: Health Source Type: news
Long non-coding RNA H19 (lncRNA H19) has been implicated in tumorigenesis and metastasis of breast cancer through regulating epithelial to mesenchymal transition (EMT); however, the underlying mechanisms remai...
Source: Cancer Cell International - Category: Cancer & Oncology Authors: Tags: Primary research Source Type: research
This study highlights the importance of global AS regulation during EMT in cancer progression and paves the way for further investigation into RNA regulation in EMT and metastasis. PMID: 32467311 [PubMed - as supplied by publisher]
Source: RNA - Category: Genetics & Stem Cells Authors: Tags: RNA Source Type: research
The RNA-binding protein Musashi-2 (MSI2) has been implicated in the tumorigenesis and tumor progression of some human cancers. MSI2 has also been reported to suppress tumor epithelial-to-mesenchymal transition...
Source: Journal of Experimental and Clinical Cancer Research - Category: Cancer & Oncology Authors: Tags: Research Source Type: research
Contributors : Pierre Bourdely ; Kristine Vaivode ; Giorgio Anselmi ; Rodrigo N Ramos ; Yoann Missolo-Kousou ; Sofia Hidalgo ; Jimena Tosselo ; Nicolas Nunez ; Wilfrid Richer ; Anne Vincent-Salomon ; Alka Saxena ; Kristie Wood ; Alvaro Lladser ; Eliane Piaggio ; Julie Helft ; Pierre GuermonprezSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensDendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Pierre Bourdely ; Kristine Vaivode ; Giorgio Anselmi ; Rodrigo N Ramos ; Yoann Missolo-Kousou ; Sofia Hidalgo ; Jimena Tosselo ; Nicolas Nunez ; Wilfrid Richer ; Anne Vincent-Salomon ; Alka Saxena ; Kristie Wood ; Alvaro Lladser ; Eliane Piaggio ; Julie Helft ; Pierre GuermonprezSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensDendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Pierre Bourdely ; Kristine Vaivode ; Giorgio Anselmi ; Rodrigo N Ramos ; Yoann Missolo-Kousou ; Sofia Hidalgo ; Jimena Tosselo ; Nicolas Nunez ; Wilfrid Richer ; Anne Vincent-Salomon ; Alka Saxena ; Kristie Wood ; Alvaro Lladser ; Eliane Piaggio ; Julie Helft ; Pierre GuermonprezSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensDendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Pierre Bourdely ; Kristine Vaivode ; Giorgio Anselmi ; Rodrigo N Ramos ; Yoann Missolo-Kousou ; Sofia Hidalgo ; Jimena Tosselo ; Nicolas Nunez ; Wilfrid Richer ; Anne Vincent-Salomon ; Alka Saxena ; Kristie Wood ; Alvaro Lladser ; Eliane Piaggio ; Julie Helft ; Pierre GuermonprezSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensDendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
CONCLUSIONS: In conclusion, SNORA71B promotes BC cells across the BBB partly via inducing EMT. PMID: 32458152 [PubMed - as supplied by publisher]
Source: Breast Cancer - Category: Cancer & Oncology Authors: Tags: Breast Cancer Source Type: research
This article provides a set of protocols for generating uniform spheroids with a thin sheet of basement membrane for studying the initial invasion of mammary epithelial cells into a surrounding collagen-rich extracellular matrix. Details are provided for generating 3D spheroids with a basement membrane, polymerizing collagen I, embedding the spheroids in the 3D collagen gel, and immunostaining the spheroids for invasion studies. Published 2020. U.S. Government. Basic Protocol 1: Growth of uniformly sized tumor spheroids with an encapsulating basement membrane Basic Protocol 2: Polymerization and embedding of tumor spheroid...
Source: Current Protocols in Cell Biology - Category: Cytology Authors: Tags: Curr Protoc Cell Biol Source Type: research
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