Intratumoral heterogeneity of glutaminase and lactate dehydrogenase A protein expression in colorectal cancer.
Intratumoral heterogeneity of glutaminase and lactate dehydrogenase A protein expression in colorectal cancer.
Oncol Lett. 2020 Apr;19(4):2934-2942
Authors: Mizuno Y, Hattori K, Taniguchi K, Tanaka K, Uchiyama K, Hirose Y
Abstract
The high expression of metabolic enzymes, including glutaminase (GA) and lactate dehydrogenase A (LDHA), which contribute to bioenergetics and biosynthesis of mammalian cells, has been identified in a variety of cancer types. The current study indicated intratumoral heterogeneity with respect to protein expression of the metabolic enzymes in colorectal cancer (CRC). GA protein expression was determined using immunohistochemistry in 98 cases of surgically resected T3 CRC. A total of 75 cases (74%) exhibited moderate to strong immunopositivity of GA based on whole-section examination. A significant correlation was demonstrated between GA expression and clinicopathological features, including histological type and tumor budding in a patient population. Detailed histological analysis revealed the upregulation of GA protein expression at the invasive margin, including tumor budding of CRC tissues. Semi-quantitative examination revealed a significant difference in immunoexpression level of GA between the invasive margin and central CRC. However, LDHA expression exhibited an opposite pattern, with expression elevated at the center and significantly decreased at the tumors invasive margin. Immunohistochemical expre...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research