Methylation profiling in promoter sequences of ATM and CDKN2A (p14ARF/p16INK4a ) genes in blood and cfDNA from women with impalpable breast lesions.

The objective of the present study was to evaluate the epigenetic changes occurring in early stages of breast cancer. The present study investigated the methylation profile of the ATM, p14ARF and p16INK4a promoters in total blood and plasma cell-free DNA (cfDNA) from women with impalpable breast lesions compared with in total blood of a control cohort of women without breast lesions. The samples were evaluated using the methylation-specific PCR method. The Fisher's exact test was used to evaluate statistical significance between the methylation and clinical variables. A total of 111 women were evaluated, including 56 women with impalpable breast cancer (39/56 also had paired plasma cfDNA) and 55 women in the control cohort (55 blood DNA). For blood DNA from women with malignant impalpable breast lesions, p16INK4a exhibited the greatest percentage of methylation (48%), followed by ATM (37.5%) and p14ARF (27%) promoters, regardless of age variation. For plasma cfDNA, the methylation rates for ATM, p14ARF and p16INK4a were 26, 26 and 10%, respectively. The methylation rates for the blood DNA of controls were the lowest for ATM (9%), p14ARF (7%) and p16INK4a (7%). The women with impalpable breast lesions (benign and malignant lesions) exhibited the highest methylation rate, regardless of age, compared with the paired plasma cfDNA and controls. This epigenetic change was statistically significant for the promoters of ATM (P=0.009) and p16INK4a (P=0.001) (impalpable breast lesions ...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Related Links:

Publication date: Available online 30 May 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Deborah Delbue, Bruna S. Mendonça, Marcela C. Robaina, Lauana G.T. Lemos, Pedro I. Lucena, João P.B. Viola, Lídia M. Magalhães, Susanne Crocamo, Caio A.B. Oliveira, Felipe R. Teixeira, Raquel C. Maia, Gabriela Nestal de Moraes
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - Category: Molecular Biology Source Type: research
Publication date: Available online 31 May 2020Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Camila A.P. Monteiro, Aline D.P.R. Oliveira, Ryan C. Silva, Rennan R.M. Lima, Fabricio O. Souto, Mariana O. Baratti, Hernandes F. Carvalho, Beate S. Santos, Paulo E. Cabral Filho, Adriana Fontes
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
Publication date: June 2020Source: European Journal of Surgical Oncology, Volume 46, Issue 6Author(s): Aoife Sartini-Bhreathnach, Edward Murphy, Siun Walsh, Malcolm Kell, Mitchell Barry, Claire Smith, Maurice Stokes
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
Publication date: June 2020Source: European Journal of Surgical Oncology, Volume 46, Issue 6Author(s): Rebecca L. Wilson, George Boundouki, Julia R. Henderson, Paula Duxbury, James R. Harvey, Julie Wray, Rajiv Dave, Cliona C. Kirwan
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
Publication date: June 2020Source: European Journal of Surgical Oncology, Volume 46, Issue 6Author(s): Vicki Kiesel, Gordon Wishart
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
Publication date: June 2020Source: European Journal of Surgical Oncology, Volume 46, Issue 6Author(s): Katherine Fairhurst, Richard Swan, Charlotte Grant, Nicola Laurence
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
Publication date: June 2020Source: European Journal of Surgical Oncology, Volume 46, Issue 6Author(s): Eleanor Walder, Elizabeth Cox
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
Publication date: June 2020Source: European Journal of Surgical Oncology, Volume 46, Issue 6Author(s): Robyn Reddington, Morgan Galer, Amanda Hagedorn, Ehab Husain, Ravi Sharma, Valerie Speirs, Yazan Masannat
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
Publication date: June 2020Source: European Journal of Surgical Oncology, Volume 46, Issue 6Author(s): Natalia Roszkowski, Selina Lam, Ellen Copson, Ramsey Cutress, Rachel Oeppen
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
Publication date: June 2020Source: European Journal of Surgical Oncology, Volume 46, Issue 6Author(s): Rudradeep Banerjee, Diptendra Kumar Sarkar
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
More News: Breast Cancer | Cancer | Cancer & Oncology | Genetics | Statistics | Study | Women