Electroacupuncture Relieves Suppression of Autophagy in Interstitial Cells of Cajal of Diabetic Gastroparesis Rats.

In this study, we aimed to figure out the relationship between DGP and autophagy of ICCs. Methods: Sixty Sprague-Dawley (SD) rats were randomly divided into normal control group (NC, 10) and modeling group (50). Rats in the modeling group were injected 2% streptozotocin (STZ) and fed with high-glucose and high-fat diet for 8 weeks in order to establish DGP rat model. After modeling, 30 successfully modeled rats were randomly selected and separated into diabetic gastroparesis group (DGP, 10), GDP rats with electroacupuncture group (EA, 10), and GDP rats with metoclopramide group (MP, 10). When the intervention was completed, blood glucose was measured by ONE TOUCH glucometer and gastrointestinal propulsive rate was detected through measuring optical density. Autophagosomes were observed under transmission electron microscope (TEM). The expression of LC3 protein and P62 protein was measured by Western blot. When ICCs were transfected with GFP-RFP-LC3 plasmid, autophagy flux was observed by laser scanning confocal microscope. Results: (1) After intervention, compared with blood glucose of rats in the NC group, all of the DGP, EA, and MP groups were remarkably increased (P
Source: Canadian Journal of Gastroenterology - Category: Gastroenterology Tags: Can J Gastroenterol Hepatol Source Type: research

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Source: Circulation Research - Category: Cardiology Authors: Tags: Circ Res Source Type: research
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Source: Cadernos de Saude Publica - Category: International Medicine & Public Health Authors: Tags: Cad Saude Publica Source Type: research
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Source: Journal of Diabetes Investigation - Category: Endocrinology Authors: Tags: CASE REPORT Source Type: research
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Source: Radiologia - Category: Radiology Authors: Tags: Radiologia Source Type: research
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Source: Diabetes Research and Clinical Practice - Category: Endocrinology Authors: Tags: Commentary Source Type: research
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Source: Journal of Ophthalmology - Category: Opthalmology Tags: J Ophthalmol Source Type: research
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Source: Medicina (Kaunas) - Category: Universities & Medical Training Authors: Tags: Medicina (Kaunas) Source Type: research
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Source: Johnson and Johnson - Category: Pharmaceuticals Tags: Innovation Source Type: news
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Source: Neural Plasticity - Category: Neurology Authors: Tags: Neural Plast Source Type: research
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