Role of complement 3 in renin generation during the differentiation of mesenchymal stem cells to smooth muscle cells.

Role of complement 3 in renin generation during the differentiation of mesenchymal stem cells to smooth muscle cells. Am J Physiol Cell Physiol. 2020 Mar 25;: Authors: Chen L, Fukuda N, Shimizu S, Kobayashi H, Tanaka S, Nakamura Y, Matsumoto T, Abe M Abstract In the present study,to investigate contributions of complement 3 (C3)to development of the pathogenesis of hypertension, we evaluated theformationof renin-producing cells and roles of C3 in renin generation during differentiationof primarybone marrow-mesenchymal stem cells (MSCs) from C57BL/6 mice, Wistar-Kyoto(WKY) rats and spontaneously hypertensive rats (SHRs)to smooth muscle cells (SMCs)with transforming growth factor-β1.The expression of renin transiently increased with increases in transcription factor liver X receptor a(LXRa), and expression of C3 and Krüppel-like factor5 (KLF5)increased during differentiation of MSCs from C57BL/6 mice, WKY rats and SHRsto SMCs. Exogenous C3a stimulated reninand LXRaexpression accompanied by nuclear translocation of LXRα. C3a receptor antagonist SB290157 suppressed renin and LXRα expression with inhibition of nuclear translocation of LXRα during the differentiation of mouse MSCs to SMCs. The expression of C3 andKLF5was significantly higher in the differentiated cellsfrom SHRs compared to cells from WKY rats during differentiation. Renin-producing cellswere formed during differentiationof MSCsandrenin generation was observed in undif...
Source: American Journal of Physiology. Cell Physiology - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research