High protection of mice against Brucella abortus by oral immunization with recombinant probiotic Lactobacillus casei vector vaccine, expressing the outer membrane protein OMP19 of Brucella species.

In this study, the gene encoding OMP19 antigen was primarily amplified and cloned into an expression vector called pT1NX, and then transformed to L. casei cell via electroporation technique. The expression was confirmed using specific antibody against the recombinant protein via immunological screening tests such as western blot and immunofluorescence assay. Finally, recombinant L. casei was orally fed to mice and the results were further recorded, indicating that the mice group which received OMP19 through L. casei based vaccine represented a very good general and mucosal immune responses protective against challenges with virulent B. abortus 544 strain compared with negative control recipient groups. Therefore, the vaccine produced in this research plan can be a very good candidate for protection against brucellosis. PMID: 32208191 [PubMed - as supplied by publisher]
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research

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by Carine Rodrigues Pereira, Jo ão Vitor Fernandes Cotrim de Almeida, Izabela Regina Cardoso de Oliveira, Luciana Faria de Oliveira, Luciano José Pereira, Márcio Gilberto Zangerônimo, Andrey Pereira Lage, Elaine Maria Seles Dorneles Brucellosis is a neglected zoonotic disease of remarkable importance worldwide. The focus of this systematic review was to investigate occupational brucellosis and to identify the main infection risks for each group exposed to the pathogen. Seven databases were used to identify papers related to o ccupational brucellosis: CABI, Cochrane, Pubmed, Scielo, Science Direc...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
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Source: EcoHealth - Category: Environmental Health Source Type: research
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Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
In this study, we evaluated efficacy of two novel multi-peptide vaccine candidates of FliC, 7α-HSDH, BhuA antigens with and without poly I:C adjuvant. Hence, humoral and cellular immune responses and protective efficacy were determined in immunized mice. Our investigation indicated that multi-epitope antigens showed a significant induction of Th1 immunity with high levels of specific IgG (especially the IgG2a), as well as IFN-γ and IL-2 compared to the control group. The addition of poly I:C to multi-epitope antigens improved the humoral and cellular immune responses. The multi-epitope antigens with and without...
Source: International Immunopharmacology - Category: Allergy & Immunology Authors: Tags: Int Immunopharmacol Source Type: research
In this study, we employed a reverse vaccinology strategy to compute the core proteome of 90 completed genomes: 55 B. melitensis, 17 B. abortus, and 18 B. suis. The core proteome was analyzed by a metasubcellular localization prediction pipeline to identify surface-associated proteins. The identified proteins were thoroughly analyzed using various in silico tools to obtain the most potential protective antigens. The number of core proteins obtained from analyzing the 90 proteomes was 1939 proteins. The surface-associated proteins were 177. The number of potential antigens was 87; those with adhesion score ≥&thins...
Source: Journal of Immunology Research - Category: Allergy & Immunology Tags: J Immunol Res Source Type: research
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Source: African Journal of Reproductive Health - Category: African Health Tags: Afr J Reprod Health Source Type: research
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Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
Background: Brucellosis is an important zoonotic disease causing huge economic losses to livestock industry and human sufferings worldwide. Current treatment of bovine Brucellosis is ineffective and infected animals become carriers. Brucella abortus Strains S19, producing antibody alone and RB51, inducing cell mediated immunity only, are used as live attenuated vaccines. However, antibody alone is ineffective for the intracellular pathogen and RB51 is Rifampicin resistant. Both strains can infect humans and cause abortions in animals.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: UMP. 826 Source Type: research
Abstract Brucellosis is an infectious disease caused by Brucella bacteria that cause disease in animals and humans. Brucellosis is one of the most common zoonotic diseases transmitted animals -to-human through direct contact with infected animals and also consumption of unpasteurized dairy products. Due to wide incidence of brucellosis in Iran and economical costs in industry animal husbandry, Vaccination is best way to prevent of this disease. All of available commercial vaccines against brucellosis are derived from live attenuated strains of Brucella. But because of disadvantage of live attenuated vaccines, prot...
Source: Infectious Disorders Drug Targets - Category: Infectious Diseases Authors: Tags: Infect Disord Drug Targets Source Type: research
tilde;ate A Abstract Brucella abortus is the etiological agent of brucellosis, a zoonotic disease affecting cattle and humans. This disease has been partially controlled in cattle by immunization with live attenuated B. abortus S19 and RB51 strains. However, use of these vaccine strains has been associated with safety issues in animals and humans. New vaccines have since emerged in the prevention of brucellosis, particularly DNA vaccines, which have shown effectiveness and a good safety profile. Their protection efficacy in mice is associated with the induction of Th1 type and cytotoxic T cell mediated immune resp...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
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