Non ‐enzymatic oxygenated metabolite of docosahexaenoic acid, 4(RS)‐4‐F4t‐neuroprostane, acts as a bioactive lipid molecule in neuronal cells

In this study, 4‐F4t‐NeuroP was introduced into neuroblastoma cells (SH‐SY5Y) and, byin vivo infusion, into rodents. Targeted lipidomic analysis of liver and brain tissues show significant elevation of anti ‐inflammatory hydroxylated‐DHA metabolites and an isomer of neuroprotectin D1, suggesting potential beneficial bioactivities of 4‐F4t‐NeuroP. Additionally, 4‐F4t‐NeuroP treatment in SH‐SY5Y cells and primary neuronal culture consistently upregulates the transcriptional level of the antioxidant enzyme hemeoxygenase‐1, but the effect is reduced when 4‐F4t‐NeuroP is further oxidized. Our data suggest that 4‐F4t‐NeuroP could be neuroprotective in the native state but may have disadvantageous bioactivity when oxidized extensively.
Source: FEBS Letters - Category: Biochemistry Authors: Tags: COMMUNICATION Source Type: research