GSE147548 The Epigenomic Landscapeof PituitaryAdenomas Reveals Specific Alterations and Differentiates Among Acromegaly, Cushing's Disease and Endocrine-Inactive Subtypes

Contributors : Matthew P Salomon ; Chikako Matsuba ; Garni Barkhoudarian ; Daniel F Kelly ; Dave S HoonSeries Type : Methylation profiling by arrayOrganism : Homo sapiensRecurrent single-nucleotide and small indel somatic mutations were infrequent among the three adenoma subtypes. However, somatic copy-number alterations (SCNA) were identified in all three pituitary adenoma subtypes. Methylation analysis revealed adenoma subtype-specific DNA methylation profiles, with GHsecreting adenomas being dominated by hypomethylated sites. Likewise, gene-expression patterns revealed adenoma subtype-specific profiles. Integrating DNA methylation and gene-expression data revealed that hypomethylation of promoter regions are related with increased expression of GH1 and SSTR5 genes in GH-secreting adenomas and POMC gene in ACTH secreting adenomas. Finally, multispectral IHC staining of immune-related proteins showed abundant expression of PD-L1 among all three adenoma subtypes.

Source: GEO: Gene Expression Omnibus - March 26, 2020 Category: Genetics & Stem Cells Tags: Methylation profiling by array Homo sapiens Source Type: research