Molecules, Vol. 25, Pages 1478: New Substituted Benzoylthiourea Derivatives: From Design to Antimicrobial Applications

Molecules, Vol. 25, Pages 1478: New Substituted Benzoylthiourea Derivatives: From Design to Antimicrobial Applications Molecules doi: 10.3390/molecules25071478 Authors: Carmen Limban Mariana Carmen Chifiriuc Miron Teodor Caproiu Florea Dumitrascu Marilena Ferbinteanu Lucia Pintilie Amalia Stefaniu Ilinca Margareta Vlad Coralia Bleotu Luminita Gabriela Marutescu Diana Camelia Nuta The increasing threat of antimicrobial resistance to all currently available therapeutic agents has urged the development of novel antimicrobials. In this context, a series of new benzoylthiourea derivatives substituted with one or more fluorine atoms and with the trifluoromethyl group have been tested, synthesized, and characterized by IR, NMR, CHNS and crystal X-ray diffraction. The molecular docking has provided information regarding the binding affinity and the orientation of the new compounds to Escherichia coli DNA gyrase B. The docking score predicted the antimicrobial activity of the studied compounds, especially against E. coli, which was further demonstrated experimentally against planktonic and biofilm embedded bacterial and fungal cells. The compounds bearing one fluorine atom on the phenyl ring have shown the best antibacterial effect, while those with three fluorine atoms exhibited the most intensive antifungal activity. All tested compounds exhibited antibiofilm activity, correlated with the trifluoromethyl substituent, most favorable in para position.
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research