Reliability factor for identification of amylolytic enzyme activity in the optimized starch-iodine assay.

Reliability factor for identification of amylolytic enzyme activity in the optimized starch-iodine assay.
Anal Biochem. 2020 Mar 19;:113696
Authors: Gaenssle ALO, van der Maarel MJEC, Jurak E
Abstract
Amylolytic enzymes are a group of proteins degrading starch to its constitutional units. For high-throughput screening, simple yet accurate methods in addition to the reducing ends assays are required. In this article, the iodine assay, a photometric assay based on the intensely colored starch-iodine complex, was adapted to enable accurate and objective differentiation between enzyme and background activity using a newly introduced mathematical factor. The method was further improved by designing a simple setup for multiple time point detection and discussing the applicability of single wavelength measurements.
PMID: 32201136 [PubMed - as supplied by publisher]

Source: Analytical Biochemistry - March 18, 2020 Category: Biochemistry Authors: Gaenssle ALO, van der Maarel MJEC, Jurak E Tags: Anal Biochem Source Type: research