Chemical and Colloidal Dynamics of MnO2 Nanosheets in Biological Media Relevant for Nanosafety Assessment

Manganese dioxide (MnO2) nanosheets undergo complex reductive dissolution in the presence of diverse biomolecular reducing agents. When dissolution in cell culture media is slow, intact nanosheets can enter cells, where they can deplete intracellular glutathione and induce cytotoxicity through intracellular Mn2+ release. The results are used to classify MnO2 nanosheets within a new biopersistence ‐based hazard screening framework for 2D materials. AbstractMany layered crystal phases can be exfoliated or assembled into ultrathin 2D nanosheets with novel properties not achievable by particulate or fibrous nanoforms. Among these 2D materials are manganese dioxide (MnO2) nanosheets, which have applications in batteries, catalysts, and biomedical probes. A novel feature of MnO2 is its sensitivity to chemical reduction leading to dissolution and Mn2+ release. Biodissolution is critical for nanosafety assessment of 2D materials, but the timing and location of MnO2 biodissolution in environmental or occupational exposure scenarios are poorly understood. This work investigates the chemical and colloidal dynamics of MnO2 nanosheets in biological media for environmental and human health risk assessment. MnO2 nanosheets are insoluble in most aqueous phases, but react with strong and weak reducing agents in biological fluid environments. In vitro, reductive dissolution can be slow enough in cell culture media for MnO2 internalization by cells in the form of intact nanosheets, which loca...
Source: Small - Category: Nanotechnology Authors: Tags: Full Paper Source Type: research