Advances in Nanomaterial-Based Microwaves and Infrared Wave-Assisted Tryptic Digestion for Utrafast Proteolysis and Rapid Detection by MALDI-MS.

Advances in Nanomaterial-Based Microwaves and Infrared Wave-Assisted Tryptic Digestion for Utrafast Proteolysis and Rapid Detection by MALDI-MS.
Comb Chem High Throughput Screen. 2013 Nov 10;
Authors: Kailasa SK, Hui-Fen W
Abstract
The unique physico-chemical properties of nanomaterials have significantly impacted in electromagnetic waves (microwave and infrared waves)-assisted tryptic digestion approaches by using them as heat absorbers to expedite digestion and as affinity probes to enrich digested proteins prior to MALDI-MS analysis. We review recent developments in electromagnetic waves (microwaves and infrared waves)-assisted proteolysis using nanomaterials as heat absorbers and as affinity probes for analysis of digested proteins in MALDI-MS. New trends in ultrafast proteolysis (nonphosphoproteins- lysozyme, cytochrome c, myoglobin and bovine serum albumin (BSA); phosphoproteins- α- and β-caseins) using nanomaterials-based microwaves- and infrared (IR) waves-assisted digestion approaches for rapid identification of digested proteins by MALDI-MS.
PMID: 24206112 [PubMed - as supplied by publisher]

Source: Combinatorial Chemistry and High Throughput Screening - November 10, 2013 Category: Chemistry Authors: Kailasa SK, Hui-Fen W Tags: Comb Chem High Throughput Screen Source Type: research