Interaction between Bax and Bcl-XL proteins confirmed by partial acceptor photobleaching-based FRET imaging

In this report, we implemented pixel-to-pixel PbFRET imaging on a wide-field microscope to map the FRET efficiency ([math] images of single living HepG2 cells co-expressing CFP-Bax and YFP-Bcl-XL. The [math] value between CFP-Bax and YFP-Bcl-XL was 4.59% in cytosol and 11.31% on mitochondria, conclusively indicating the direct interaction of the two proteins, and the interaction of the two proteins was strong on mitochondria and modest in cytosol.

Source: Journal of Innovative Optical Health Sciences - February 25, 2020 Category: Biomedical Science Authors: Fangfang Yang Mengyan Du Xiaoping Wang Tongsheng Chen Source Type: research