Evaluation of in vivo anti-inflammatory and anti-angiogenic attributes of methanolic extract of Launaea spinosa

This study was conducted to evaluate anti-inflammatory and anti-angiogenic activities of methanol extract ofLaunaea spinosa. Extraction was performed by maceration and the resultant green coloured extract was labelled asLs.Me. Solubility analysis showed thatLs.Me was miscible with distilled water, normal saline, ethanol and methanol. Metal analysis following acid digestion method exhibited the presence of copper, magnesium, manganese, iron, zinc and calcium. Phytochemical analysis confirmed the presence of different classes of secondary metabolites inLs.Me. HPLC analysis showed the presence of quercetin, gallic acid, caffeic acid, benzoic acid and sinapic acid inLs.Me. Data of in vitro antioxidant assays showed moderate antioxidant potential ofLs.Me which was also confirmed by data of in vivo enzymes (SOD, CAT, and TSP)  assays. Antimicrobial assays data showed thatLs.Me was active againstS.aureus andS.epidermidis (bacterial) as well asC.albicans andA.niger (fungal) strains. Data of acute physio-pathological studies showed no abnormalities in Albino rats up to the dose of 2000  mg/kg ofLs.Me. Acute and chronic inflammatory models were used to evaluate anti-inflammatory effects ofLs.Me. Data of acute studies showed thatLs.Me has the potential to arrest inflammation produced by different mediators in a dose-dependent manner. 200  mg/kg ofLs.Me was found to produce significantly (p <  0.05) better anti-inflammatory effects than 100 mg/kg ofLs.Me.Ls.Me also significan...
Source: Inflammopharmacology - Category: Drugs & Pharmacology Source Type: research