Mutational diversity in mutY deficient Helicobacter pylori and its effect on adaptation to the gastric environment.

Mutational diversity in mutY deficient Helicobacter pylori and its effect on adaptation to the gastric environment. Biochem Biophys Res Commun. 2020 Mar 09;: Authors: Kinoshita-Daitoku R, Kiga K, Sanada T, Ogura Y, Bo Z, Iida T, Yokomori R, Kuroda E, Tanaka M, Sood A, Suzuki T, Nakai K, Hayashi T, Mimuro H Abstract Helicobacter pylori, a pathogenic bacterium that colonizes in the human stomach, harbors DNA repair genes to counter the gastric environment during chronic infection. In addition, H. pylori adapts to the host environment by undergoing antigenic phase variation caused by genomic mutations. The emergence of mutations in nucleotide sequences is one of the major factors underlying drug resistance and genetic diversity in bacteria. However, it is not clear how DNA repair genes contribute to driving the genetic change of H. pylori during chronic infection. To elucidate the physiological roles of DNA repair genes, we generated DNA repair-deficient strains of H. pylori (ΔuvrA, ΔuvrB, ΔruvA, Δnth, ΔmutY, ΔmutS, and Δung). We performed susceptibility testing to rifampicin in vitro and found that ΔmutY exhibited the highest mutation frequency among the mutants. The number of bacteria colonizing the stomach was significantly lower with ΔmutY strain compared with wild-type strains in a Mongolian gerbil model of H. pylori infection. Furthermore, we performed a genomic sequence analysis of the strains isolated from the Mong...
Source: Biochemical and Biophysical Research communications - Category: Biochemistry Authors: Tags: Biochem Biophys Res Commun Source Type: research