Innovation by homologous recombination.

Innovation by homologous recombination.
Curr Opin Chem Biol. 2013 Oct 29;
Authors: Trudeau DL, Smith MA, Arnold FH
Abstract
Swapping fragments among protein homologs can produce chimeric proteins with a wide range of properties, including properties not exhibited by the parents. Computational methods that use information from structures and sequence alignments have been used to design highly functional chimeras and chimera libraries. Recombination has generated proteins with diverse thermostability and mechanical stability, enzyme substrate specificity, and optogenetic properties. Linear regression, Gaussian processes, and support vector machine learning have been used to model sequence-function relationships and predict useful chimeras. These approaches enable engineering of protein chimeras with desired functions, as well as elucidation of the structural basis for these functions.
PMID: 24182747 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - October 29, 2013 Category: Biochemistry Authors: Trudeau DL, Smith MA, Arnold FH Tags: Curr Opin Chem Biol Source Type: research