Apolipoprotein A-IV enhances fatty acid uptake by adipose tissues of male mice via sympathetic activation.

Apolipoprotein A-IV enhances fatty acid uptake by adipose tissues of male mice via sympathetic activation. Endocrinology. 2020 Mar 10;: Authors: Zhu Q, Weng J, Shen M, Fish J, Shen Z, Coschigano KT, Davidson WS, Tso P, Shi H, Lo CC Abstract Apolipoprotein A-IV (ApoA-IV) synthesized by the gut regulates lipid metabolism. Sympathetic innervation of adipose tissues also controls lipid metabolism. We hypothesized that ApoA-IV required sympathetic innervation to increase fatty acid (FA) uptake by adipose tissues and brown adipose tissue (BAT) thermogenesis. After 3 weeks feeding of either a standard chow diet or a high-fat diet (HFD), mice with unilateral denervation of adipose tissues received intraperitoneal administration of recombinant ApoA-IV protein and intravenous infusion of lipid mixture with radioactive triolein. In chow-fed mice, ApoA-IV administration increased FA uptake by intact BAT, but not the contralateral denervated BAT or intact white adipose tissue (WAT). Immunoblots showed that, in chow-fed mice, ApoA-IV increased expression of lipoprotein lipase (LPL) and tyrosine hydroxylase (TH) in both intact BAT and inguinal WAT (IWAT); while ApoA-IV enhanced protein levels of β3 adrenergic receptor (β3-AR), adipose triglyceride lipase (ATGL), and uncoupling protein 1 (UCP1) in the intact BAT only. In HFD-fed mice, ApoA-IV elevated FA uptake by intact epididymal WAT (EWAT), but not intact BAT or IWAT. ApoA-IV increased sympathe...
Source: Endocrinology - Category: Endocrinology Authors: Tags: Endocrinology Source Type: research