Insufficient CD100 shedding contributes to suppression of CD8+ T cell activity in non-small cell lung cancer.

Insufficient CD100 shedding contributes to suppression of CD8+ T cell activity in non-small cell lung cancer. Immunology. 2020 Mar 09;: Authors: Wang HM, Zhang XH, Ye LQ, Zhang K, Yang NN, Geng S, Chen J, Zhao SX, Yang KL, Fan FF Abstract CD100 is an immune semaphorin constitutively expressed on T cells. Matrix metalloproteinase (MMP) is an important mediator of membrane-bound CD100 (mCD100) cleavage to generate soluble CD100 (sCD100), which has immunoregulatory activity in immune cell responses. The aim of the study was to investigate the level and role of sCD100 and mCD100 in modulating CD8+ T cell function in non-small cell lung cancer (NSCLC). sCD100 and MMP-14 levels in the serum and bronchoalveolar lavage fluid (BALF), and mCD100 expression on peripheral and lung-resident CD8+ T cells were analysed in NSCLC patients. The ability to induce sCD100 and the effect of MMP-14 on mCD100 shedding for the regulation of noncytolytic and cytolytic functions of CD8+ T cells were also analysed in direct and indirect contact coculture systems. NSCLC patients had lower serum sCD100 and higher mCD100 levels on CD8+ T cells compared with healthy controls. BALF from the tumor site also had decreased sCD100 and increased mCD100 on CD8+ T cells compared with the nontumor site. Recombinant CD100 stimulation enhanced noncytolytic and cytolytic functions of CD8+ T cells from NSCLC patients, whereas blockade of CD100 receptor CD72 attenuated CD8+ T ce...
Source: Immunology - Category: Allergy & Immunology Authors: Tags: Immunology Source Type: research